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Enzyme Linked immunosorbent Assay Video Lecture | Crash Course for CSIR NET Life Sciences
FAQs on Enzyme Linked immunosorbent Assay Video Lecture - Crash Course for CSIR NET Life Sciences
| 1. What is the principle behind the Enzyme Linked Immunosorbent Assay (ELISA)? |  |
Ans. The principle of ELISA is based on the specific binding of an antigen to an antibody. In this assay, a target antigen is immobilized on a solid surface, and a specific enzyme-linked antibody is applied. The enzyme reacts with a substrate to produce a measurable signal, often a color change, which correlates with the amount of antigen present in the sample.
| 2. What are the different types of ELISA? | |
Ans. There are several types of ELISA, including direct ELISA, indirect ELISA, sandwich ELISA, and competitive ELISA. Direct ELISA uses a single enzyme-linked antibody for detection, while indirect ELISA involves a primary antibody and a secondary enzyme-linked antibody. Sandwich ELISA captures the target antigen between two antibodies, and competitive ELISA measures the concentration of an antigen by its ability to compete with a labeled antigen for binding sites on an antibody.
| 3. What are the common applications of ELISA in research and clinical settings? | |
Ans. ELISA is widely used in both research and clinical laboratories for various applications, such as detecting and quantifying proteins, hormones, antibodies, and other biomolecules in samples. It is commonly employed in diagnostics for diseases, monitoring immune responses, and in vaccine development to assess antibody levels.
| 4. What are the advantages of using ELISA over other immunoassays? | |
Ans. ELISA offers several advantages, including high sensitivity and specificity, the ability to process multiple samples simultaneously, and a relatively simple procedure that does not require sophisticated equipment. Additionally, the quantitative nature of ELISA allows for precise measurement of analyte concentrations, making it a preferred choice for many applications.
| 5. What are the key steps involved in performing an ELISA? | |
Ans. The key steps in performing an ELISA include coating a microplate with the antigen, blocking non-specific binding sites, adding the sample containing the target analyte, applying a specific enzyme-linked antibody, washing to remove unbound antibodies, adding a substrate for the enzyme, and finally measuring the signal produced, typically using a spectrophotometer.
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Sep 28, 2025
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