Photosynthesis (Part - 2) - Photosynthesis in Higher Plants, Biology, Class 11 PDF Download

Light reactions, Photo Systems- photosynthesis in higher plants, Biology, Class 11

MECHANISM OF PHOTOSYNTHESIS

Light reaction/Hill reaction/Photochemical reaction/Generation of assimilatory powers (NADPH2 +  ATPs)/Photophase.

• Antenna or accessory pigments receive radient energy and transfer it among themselves. This transfer of energy is known as resonance transfer. Then antenna molecules excited and transfer their energy to the chlorophyll 'a' molecules of reaction centre.

It is known as inductive resonance.Finally chl. 'a' of leaf center molecules converts the light energy into electrical energy by bringing aboutelectric charge separation.

Cyclic ETS and Photophosphorylation

•  In cyclic ETS, only PS–I (LHC–I) works, which consists of Chl–'a' 670, Chl–a–683, Chl–'a'–695, carotenoids, some molecules of chl–'b' & reaction centre–Chl–'a'–700/P–700.

•  Cyclic ETS OR PS–I is activated by wavelength of light greater than 680 nm.

•  It occurs at grana thylakoids and stroma thylakoids.

•  During Cyclic ETS the electron ejected from reaction centre of PS-I, returns back to its reaction centre.

•  In cyclic ETS, no oxygen evolution occurs, because photolysis of water is absent.

•  NADPH2 (reducing power) is not formed in cyclic process.

•  Plastocyanin (PC) is Cu–containing blue coloured protein in cyclic ETS.

•  According to modern researches, first e– acceptor is FRS (Ferredoxin Reducing Substance), which is a Fe-S containing Protein. Earlier fd (Ferredoxin) was considerd as first e– acceptor.

•  Phosphorylation takes place at two places, thus two ATP generates in each cyclic ETS

•  Both PS–I and PS–II involved in non cyclic ETS.

•  PS–II (P–680) consists of Chl–a–660,  Chl–a–673, Chl–a–680, Chl–a–690, Chl–b, or Chl–c or Chl–d, carotenoids & phycobilins. Phycobilins present only in PS II

•  It occurs at grana thylakoids only.

•  The e– ejected from PS–II never back to chl–a–680 (reaction centre) & finally gained by NADP. Thus gap of e– in PS–II is filled by photolysis of water as a result, oxygen evolution occurs in Z–scheme. 

• Each turn of non cyclic ETS produces 1 ATP and 2NADPH₂ (4 mol. of water is photolysed and 1 O₂ released)

 Non cyclic Photophosphorylation/Z-scheme

•  12 NADPH₂ + 18 ATP are required as assimilatory power to produce one molecule of Glucose in dark reaction, thus6 turns of Z–scheme are necessary for the production of one glucose molecule by calvin cycle.

•  Additional 12 ATP come from 6 turn of cyclic ETS. (over all 54 ATP equivalents)

•  Primary e– acceptor in non–cyclic reaction is PQ or plastoquinone. Recently pheophytin (structure like chl. a without Mg) is considered as Ist e– acceptor in Z–scheme.

•  Plastocyanin (PC) is link between PS–I and PS–II in non cyclic ETS. (Some scientists–cyto-f)

•  Final e– acceptor in Z–scheme is NADP+ (Hill reagent)

•  During Non-Cyclic ETS energy flow takes place from PS II to PS I.

===========================================================

Chemiosmotic Hypothesis and Calvin Cycle - Photosynthesis in Higher Plants, Biology, Class 11

Photophosphorylation :

Chemiosmotic theory : Proposed by Peter Mitchell. During ETC of photosynthesis concentration of H+ gradually increases in thylakoid lumen. During cyclic photphosphrylation PQ leads to shifting of H+ from stroma to thylakoid lumen. On the other hand during non cyclic photophoshorylation threre are three causes of differential H+ ion  concentration.

(i) Photolysis of H2O produces H+

(ii) PQ shifting of H+ ion from stroma to lumen.

(iii) NADP redutase mediated utilisation of H+ from stroma.

• This differential H+ ion concentration leads to development of proton gradient and electrical potential across thylakoid memberane. Both proton gradient and electrical potential collectively called proton motive force (PMF) *

• PMF do not allow stay of H+ ions in lumen so H+ start to move towards stroma through CF0 particle selectively. The passage of 3H+ ions leads to activation of ATP synthase and forms ATP from ADP and Pi.

• Some physiologist beleive that synthesis of one ATP is required passage of 2H+ ions.

•  Blackman reaction is called as dark reaction, because no direct light is required for this. Calvin presented these reactions in cyclic manner & thus called as Calvin cycle.

• Ist stable compound of Calvin cycle is 3C–PGA (Phosphoglyceric acid) thus Calvin cycle is called as C3–cycle. (First compound is unstable, 6C keto acid)

• Study by Calvin was on Chlorella & Scenedesmus. During his experiment he used chromatography & radioisotopy (C14) techniques for detecting reactions of C3–cycle.

• Rubisco (Ribulose bis-phosphate carboxylase-oxygenase) is main enzyme in C3–cycle, which is present in stroma & it makes 16% protein of chloroplast. Rubisco is most abundant enzyme.

• CO₂–acceptor in Calvin cycle is RuBp. This carboxylation reaction is catalysed by Rubisco.

• C3, C4, C5, C6 and C7 monosaccharides are intermediates of calvin Cycle.

• C3=Phosphoglyceraldehyde and DHAP, C4=Erythrose, C5=Xylulose, Ribose, C7 = Sedoheptulose.

• The largest monosaccharide in livings are 7C–Sedoheptulose–P (Ketose)

• Warburg effect – Inhibitory effect of high conc. of O2 on photosynthesis is called as Warburg effect (It is due to Photorespiration).

•  6 turns of Calvin cycle are required for the formation of one glucose.

CalvinCycle/C3–Cycle /Reductive Pentose Phosphate Pathway, in chloroplast stroma