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Techniques: Electrophoresis Video Lecture | Crash Course for CSIR NET Life Sciences

Q: 5. What are the safety precautions that should be taken during electrophoresis experiments?

Ans. Safety precautions during electrophoresis include:1. Using Personal Protective Equipment (PPE) : Lab coats, gloves, and safety goggles should be worn to protect against chemical exposure and electrical hazards.2. Working with Low Voltage : Ensuring that the electric current is at a safe level to prevent accidents and injuries.3. Proper Handling of Chemicals : All reagents should be handled according to Material Safety Data Sheets (MSDS) to prevent exposure to hazardous substances.4. Disposing of Waste Properly : Gel and buffer waste should be disposed of according to local regulations to avoid environmental contamination.

FAQs on Techniques: Electrophoresis Video Lecture - Crash Course for CSIR NET Life Sciences

1. What is the principle of electrophoresis and how does it work?

Ans. Electrophoresis is a technique that separates charged particles in a fluid using an electric field. The principle relies on the movement of charged molecules, such as proteins or nucleic acids, towards the electrode of opposite charge. The speed of migration depends on the size, shape, and charge of the molecules. Smaller and more highly charged molecules move faster through the gel or medium, leading to separation based on these properties.

2. What are the different types of electrophoresis techniques?

Ans. There are several types of electrophoresis techniques, including: 1. Agarose Gel Electrophoresis: Used primarily for separating nucleic acids based on size. 2. Polyacrylamide Gel Electrophoresis (PAGE): Commonly used for proteins, allowing for higher resolution than agarose. 3. Isoelectric Focusing (IEF): Separates proteins based on their isoelectric point (pI). 4. Capillary Electrophoresis: Utilizes narrow capillaries to separate small volumes of samples quickly and efficiently. 5. Two-Dimensional Electrophoresis: Combines isoelectric focusing and PAGE for complex protein mixtures.

3. What role does the buffer solution play in electrophoresis?

Ans. The buffer solution in electrophoresis serves several critical functions. It maintains a stable pH during the separation process, which is vital for the integrity of the biomolecules being analyzed. The buffer also provides the necessary ions to conduct electricity, facilitating the movement of charged particles. Additionally, it helps to control the ionic strength, which can affect the resolution and efficiency of the separation.

4. How can electrophoresis be applied in the field of biotechnology?

Ans. Electrophoresis has numerous applications in biotechnology, including: 1. DNA Fingerprinting: Used in forensic science to identify individuals based on their unique DNA profiles. 2. Protein Analysis: Helps in the characterization and purification of proteins, which is essential for drug development and research. 3. Gene Studies: Assists in analyzing gene expression and mutations by separating PCR products. 4. Quality Control: Ensures the purity and integrity of biopharmaceutical products by analyzing the composition of proteins.

5. What are the safety precautions that should be taken during electrophoresis experiments?

Ans. Safety precautions during electrophoresis include: 1. Using Personal Protective Equipment (PPE): Lab coats, gloves, and safety goggles should be worn to protect against chemical exposure and electrical hazards. 2. Working with Low Voltage: Ensuring that the electric current is at a safe level to prevent accidents and injuries. 3. Proper Handling of Chemicals: All reagents should be handled according to Material Safety Data Sheets (MSDS) to prevent exposure to hazardous substances. 4. Disposing of Waste Properly: Gel and buffer waste should be disposed of according to local regulations to avoid environmental contamination.

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Sep 28, 2025 Last updated

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