CSIR NET Life Science Exam > CSIR NET Life Science Videos > Crash Course for CSIR NET Life Sciences > Thin Layer Chromatography
Thin Layer Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences
FAQs on Thin Layer Chromatography Video Lecture - Crash Course for CSIR NET Life Sciences
| 1. What is Thin Layer Chromatography (TLC) and how does it work? |  |
Ans. Thin Layer Chromatography (TLC) is a method used to separate non-volatile mixtures. In TLC, a thin layer of adsorbent material, typically silica gel or alumina, is coated on a flat, inert substrate. The sample is applied as a small spot on the layer. When a solvent (mobile phase) ascends the layer by capillary action, the components of the mixture move at different rates based on their affinities to the stationary phase (the adsorbent material) and the mobile phase. This results in the separation of the components, which can be visualized through various methods.
| 2. What are the main components involved in a TLC setup? | |
Ans. The main components of a TLC setup include a TLC plate (the stationary phase), which is typically made of glass or plastic coated with silica gel or alumina; a developing chamber, which contains the solvent (mobile phase) used for the separation; a sample application device, such as a capillary tube or a micropipette; and visualization tools, such as UV light or staining reagents to detect the separated components after development.
| 3. What factors affect the separation of compounds in TLC? | |
Ans. Several factors influence the separation of compounds in TLC, including the choice of stationary phase, the nature of the mobile phase (solvent), the thickness of the stationary phase, the temperature, and the time allowed for development. The polarity of the compounds being separated also plays a crucial role, as polar compounds interact more strongly with polar stationary phases, affecting their migration rates.
| 4. How can the results of a TLC experiment be analyzed? | |
Ans. The results of a TLC experiment can be analyzed by calculating the Retention Factor (Rf) value for each component. The Rf value is determined by measuring the distance traveled by the compound divided by the distance traveled by the solvent front. Rf = (distance traveled by the compound) / (distance traveled by the solvent front). Additionally, the separated spots can be visualized under UV light or by using staining techniques, allowing identification and comparison of the compounds based on their Rf values.
| 5. What are some common applications of Thin Layer Chromatography? | |
Ans. Thin Layer Chromatography is widely used in various fields, including chemistry, biochemistry, and pharmaceuticals. Common applications include the identification and separation of organic compounds, purity testing of substances, monitoring chemical reactions, and analyzing food products and herbal medicines. TLC is favored for its simplicity, speed, and cost-effectiveness in qualitative analysis.
About this Video
Sep 28, 2025
Last updated
Related Exams
Video Description: Thin Layer Chromatography for CSIR NET Life Science 2025 is part of Crash Course for CSIR NET Life Sciences preparation.
The notes and questions for Thin Layer Chromatography have been prepared according to the CSIR NET Life Science exam syllabus.
Information about Thin Layer Chromatography covers all important topics for CSIR NET Life Science 2025 Exam.
Find important definitions, questions, notes, meanings, examples, exercises and tests below for Thin Layer Chromatography.
Introduction of Thin Layer Chromatography in English is available as part of our Crash Course for CSIR NET Life Sciences for CSIR NET Life Science & Thin Layer Chromatography in
Hindi for Crash Course for CSIR NET Life Sciences course. Download more important topics related with notes, lectures and mock test series for
CSIR NET Life Science Exam by signing up for free.
Description
Video Lecture & Questions for Thin Layer Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences - CSIR NET Life Science full syllabus preparation | Free video for CSIR NET Life Science exam to prepare for Crash Course for CSIR NET Life Sciences.
Information about Thin Layer Chromatography
Here you can find the meaning of Thin Layer Chromatography defined & explained in the simplest way possible.
Besides explaining types of Thin Layer Chromatography theory, EduRev gives you an ample number of questions to practice Thin Layer Chromatography tests,
examples and also practice CSIR NET Life Science tests.
Related Searches
Thin Layer Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences
,Exam
,ppt
,Semester Notes
,Thin Layer Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences
,Objective type Questions
,practice quizzes
,Sample Paper
,study material
,Thin Layer Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences
,Viva Questions
,Summary
,Extra Questions
,MCQs
,Important questions
,shortcuts and tricks
,video lectures
,past year papers
,Free
,Previous Year Questions with Solutions
,mock tests for examination
;
Study Thin Layer Chromatography on the App
Students of CSIR NET Life Science can study Thin Layer Chromatography alongwith tests & analysis from the EduRev app,
which will help them while preparing for their exam. Apart from the Thin Layer Chromatography,
students can also utilize the EduRev App for other study materials such as previous year question papers, syllabus, important questions, etc.
The EduRev App will make your learning easier as you can access it from anywhere you want.
The content of Thin Layer Chromatography is prepared as per the latest CSIR NET Life Science syllabus.
|
© EduRev
|
Education Revolution
|
|
Signup to see your scores
go up within 7 days!
Access 1000+ FREE Docs, Videos and Tests
Takes less than 10 seconds to signup