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UV Visible Spectroscopy Video Lecture | Crash Course for CSIR NET Life Sciences
FAQs on UV Visible Spectroscopy Video Lecture - Crash Course for CSIR NET Life Sciences
| 1. What is UV-Visible spectroscopy and how does it work? |  |
Ans.UV-Visible spectroscopy is an analytical technique used to measure the absorbance or transmittance of ultraviolet (UV) and visible light by a sample. The process involves passing light through a sample, and as the light interacts with the molecules, certain wavelengths are absorbed. The amount of light absorbed at specific wavelengths indicates the presence and concentration of different substances within the sample.
| 2. What are the applications of UV-Visible spectroscopy in various fields? | |
Ans.UV-Visible spectroscopy is widely used in various fields such as chemistry, biology, and environmental science. It is employed for quantitative analysis of compounds, monitoring reactions, studying protein and nucleic acid interactions, and detecting pollutants in water and air samples. In the pharmaceutical industry, it is also used for drug formulation and stability testing.
| 3. What are the common instruments used in UV-Visible spectroscopy? | |
Ans.The main instrument used in UV-Visible spectroscopy is the spectrophotometer, which consists of a light source (typically a deuterium lamp for UV and a tungsten lamp for visible light), a monochromator to isolate specific wavelengths, and a detector to measure the intensity of transmitted or absorbed light. Modern spectrophotometers may also include software for data analysis and processing.
| 4. How do you prepare a sample for UV-Visible spectroscopy analysis? | |
Ans.Preparing a sample for UV-Visible spectroscopy involves ensuring that the sample is in a suitable solvent and concentration for analysis. The sample should be clear and free of any particulates that could scatter light. Typically, solutions are prepared at concentrations that fall within the linear range of the spectrophotometer to ensure accurate results.
| 5. What are the limitations of UV-Visible spectroscopy? | |
Ans.Some limitations of UV-Visible spectroscopy include its inability to detect non-absorbing species, such as certain gases and colorless solutions. Additionally, overlapping absorption spectra can complicate the analysis of complex mixtures. The technique also requires a certain level of expertise to interpret the results accurately, particularly in quantitative applications.
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Sep 28, 2025
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