Chemistry Exam > Chemistry Videos > Organic Chemistry > Working of a simple UV-Visible Spectrophotometer
Working of a simple UV-Visible Spectrophotometer Video Lecture | Organic Chemistry
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FAQs on Working of a simple UV-Visible Spectrophotometer Video Lecture - Organic Chemistry
| 1. How does a UV-Visible Spectrophotometer work? |  |
Ans. A UV-Visible Spectrophotometer works by passing a beam of ultraviolet (UV) or visible light through a sample and measuring the intensity of the transmitted or absorbed light. The instrument consists of a light source, monochromator to select the desired wavelength, a sample holder, and a detector. The sample absorbs certain wavelengths of light based on its chemical composition, and the spectrophotometer measures the amount of light absorbed, allowing for the quantification of the sample's concentration or other properties.
| 2. What are the main components of a UV-Visible Spectrophotometer? | |
Ans. The main components of a UV-Visible Spectrophotometer include a light source, monochromator, sample holder, and detector. The light source emits a broad spectrum of light, which is then passed through the monochromator. The monochromator allows for the selection of a specific wavelength of light. The selected wavelength is then directed through the sample holder, where the sample is placed. The detector measures the intensity of the transmitted or absorbed light, and this data is used to determine the properties of the sample.
| 3. How is the concentration of a sample determined using a UV-Visible Spectrophotometer? | |
Ans. The concentration of a sample can be determined using a UV-Visible Spectrophotometer by measuring the absorbance of the sample at a specific wavelength. A calibration curve is first established by measuring the absorbance of a series of standard solutions with known concentrations. The absorbance values are plotted against the corresponding concentrations, and a linear regression analysis is performed. The concentration of an unknown sample can then be determined by measuring its absorbance and using the calibration curve to find its corresponding concentration.
| 4. Can a UV-Visible Spectrophotometer be used to analyze the purity of a substance? | |
Ans. Yes, a UV-Visible Spectrophotometer can be used to analyze the purity of a substance. The purity of a substance can be determined by measuring its absorbance at a specific wavelength and comparing it to the absorbance of a pure sample of the same substance. If impurities are present, they may cause additional absorbance peaks or shifts in the spectrum, indicating the presence of other substances. By analyzing the absorbance spectrum of a sample, the purity of the substance can be assessed.
| 5. What are the advantages of using a UV-Visible Spectrophotometer? | |
Ans. Some advantages of using a UV-Visible Spectrophotometer include its wide applicability, high sensitivity, and ease of use. UV-Visible Spectrophotometers can be used to analyze a wide range of samples, including liquids, solids, and gases. They offer high sensitivity, allowing for the detection and quantification of small amounts of substances. Additionally, UV-Visible Spectrophotometers are relatively easy to operate, making them accessible to both experienced scientists and beginners in the field.
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Nov 15, 2024 Last updated |
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