Techniques MCQ - IIT JAM MCQ

As DEAE - cellulose is used that means fixed ion is positive charge in ion exchange chromatogram. So negative charge ions will be attracted and elute later.
So the P will elute first and then R and then S and finally Q.

By observing the given autoradiogram the sequence will come of amplified DNA so it will come
5’TACCTTGAT3’. So the sequence of the given DNA would be 5’ATCAAGGTA3’. 

Cetyltrimethylammonium bromide is a cationic detergent that enables quick precipitation of cell debris and other biomolecules and hence help in separation of DNA especially from plants.

Non-parametric test refer to statistical tests which do not involve numeric values (ordinal) but other indices like genetic ratios, degree of pain, etc. Chi square test is one such example. 

DNA foot printing is use to identify DNA protein interaction. Bound protein in the DNA protect cleavage of DNA by nuclease. After banding pattern analysis it can be clearly observed.

Affinity chromatography is the best method for protein purification. It is done by using specific affinity between the substrate to be isolated and molecule which can specific bind. So antigen can be purified by binding with antibody.

PCR is a technique which is use to amplify DNA invitro. It involves following steps.
(i) Denaturation at 94/95°C
(ii) Annealing at T_m± 4°
(iii) Extension at 72°C

In affinity chromatography GST-tagged fusion protein can be purified by using Glutathione as ligand.

In case of unskewed or normal data, mean, median and mode coincide.

Polyacrylamide gel electrophoresis is used to separate proteins and DNA less than 500 bp.

Electric field is dependent inversely on length of gel, it is related by the expression E = v/l, Where E is electric field, v is applied voltage and l is length of gel or distance between electrodes.

As per sigma rule a data which has normal distribution covers 68% of sample, hence weight in range of 70 ± 3 i.e. 67-73 would be covered  i.e. 153 students. While 99.5% sample coverage would be observed when we consider mean ± 3σ, i.e. 70 ±9 would cover 99.5% sample, hence number of students above and below 79 ks would be 225-  Hence 1 student would be above 79 kg and 1 below 61 kg.

Gel filtration chromatography, SDS-PAGE, MALDI-TOF mass spectroscopy all three used in the molecular weight determination while ChIP assay is used for DNA protein interaction.

2-D gel electrophoresis is used for the discovery phase in proteomics approach, whatever the proteins have been identified by 2-D gel electrophoresis then these are validates by either ELISA or western blot.

Super shift assay, DNA foot printing EMSA and ChIP assay all are used for DNA, protein interaction.

Polymerase chain reaction (PCR) is used for amplification of DNA in laboratory condition and is very much used in forensic science laboratory for application purpose. Its first step is denaturation, then annealing and  finally extension.

Dideoxy sequencing method is also known as chain termination method because after addition of dideoxy nucleotide, the growing chain terminates because of the absence of OH group in dideoxy nucleotide.

 ...(i)
 ...(ii)

M_f = m₁ x 2ⁿ
= 100 x 2⁸ 
= 100 x 256
= 25600

T_m = 4(G + C) + 2(A + T)
=4(10) + 2(10)
=40 + 20 
=60°C

Thats why17.5 Kd will be molecular weight.