Plant Tissue Culture Video Lecture | Science & Technology for UPSC CSE

hello friends I'm Shwetha Singh
assistant professor beyond Eagles
college I welcome you all on behalf of
guru kpo the topic on which I am going
to speak today is about an introduction
to plant tissue culture what plan to
show culture what are the steps and how
we use it in the form of technology so
scientists are continuously trying to
modify the domestic animals in plants
they are opting a lot of new different
type of techniques that they can use in
science so that the variety of plant or
animal that can be increased one of them
is the plant tissue culture plant tissue
culture it is a technique of culturing a
plant cell tissue or an organ in a
culturing medium in vitro or in
test-tube under laboratory condition is
septic condition so this is a definition
of plant tissue culture and there are
different type of types of plant tissue
culture now we will discuss about what
is a suspension a suspension is a liquid
formulation which is which contain
individual cells which are scattered
they are not in a collective form they
are scattered they are present in that
liquid medium so this is called as
suspension whereas a culture whereas a
callus is a mass which is collective a
cells out present collectively in a
solid media here a liquid media is not
used a solid media is used we use
relating agents like agar in case of
callus formation which occurs from an ex
plant what is an explained now explained
is the portion or you can say apart from
the original plant thus that is called
as the ex plant it can be a stem it can
be an anther it can be a shoe so that
particular part of the original plant is
called as ex plant and that expand forms
callus now another thing is about the
culture medium the medium where the ex
plant it grows to form callus word that
culture medium it contains culture
medium contains carbon sources nitrogen
sources growth regulators
like auxins and cytokinins generating
agents like agar and a pH of about five
to six point five is necessary is
necessary to maintain and a temperature
of 27 degree centigrade now auxins and
cytokinins auxins are necessary for the
growth of roots and cytokinins are
necessary for the growth of shoots
auxins like 242 for dye phenoxy acetic
acid and IA a doctors in dole acetic
acid it comes under auxins and
cytokinins like chi chi Nathan and VAP
battles benzyl amino purine so these are
the examples of these growth regulators
now what is the exact mechanism in the
plant tissue culture we take an ex plant
that explains is then allowed to grow in
the culture medium and then the callus
formation at take place that callus
formation that callus will then transfer
into a medium containing
cytokinin rich amount of cytokine a
medium where the chute will produce or
the chute will appear this process is
called as shooting then that chute will
let transfer into a medium which is
which will be having a high amount of
auxin concentration that will help in
the development of the roots when both
chute and root will develop that plant
will be taken out into the field where
that can be applied in the process of
hardening and this is how a small plant
late' is formed from one major plant
source so this is how we are using this
whole technology in the plant tissue
culture so this was all about an
introduction in plant tissue culture for
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