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Separation of Pigments from the Extract of Spinach Leaves by Paper Chromatography Video Lecture - Class 12

FAQs on Separation of Pigments from the Extract of Spinach Leaves by Paper Chromatography Video Lecture - Class 12

1. What is paper chromatography?

Ans. Paper chromatography is a technique used to separate and identify different pigments present in a mixture. It involves placing a small amount of the mixture on a strip of filter paper and then immersing the bottom of the paper in a solvent. As the solvent moves up the paper, it carries the pigments with it, creating distinct bands of separated pigments.

2. Why is spinach used in this experiment?

Ans. Spinach leaves contain various pigments, such as chlorophyll a, chlorophyll b, carotenes, and xanthophylls. These pigments can be easily extracted from spinach leaves and are ideal for demonstrating the separation technique of paper chromatography. Additionally, spinach is readily available and inexpensive, making it a convenient choice for this experiment.

3. How does paper chromatography separate pigments?

Ans. Paper chromatography separates pigments based on their solubility and affinity for the filter paper and solvent. Each pigment has a different solubility and will move at a different rate as the solvent travels up the paper. Pigments with higher solubility will move faster, while those with lower solubility will move slower. This differential movement leads to the separation of pigments into distinct bands.

4. What is the purpose of using a solvent in paper chromatography?

Ans. The solvent used in paper chromatography serves as the mobile phase that carries the pigments up the paper. It is carefully selected based on its ability to dissolve the pigments and its polarity. The choice of solvent is crucial as it determines the separation and elution of the pigments. Different solvents will result in different separation patterns, allowing for the identification of specific pigments.

5. How can the separated pigments be identified?

Ans. Once the paper chromatography is complete, the separated pigments can be identified by comparing their Rf (retention factor) values. The Rf value is calculated by dividing the distance traveled by a pigment by the distance traveled by the solvent. These values are unique for each pigment and can be compared to known Rf values to determine the identity of the pigments present in the spinach extract. Additionally, the visual appearance and color of the separated bands can provide initial clues about the pigments' identities.

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Sep 04, 2025 Last updated

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