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Spectrophotometry & Beer's Law Video Lecture | Inorganic Chemistry
FAQs on Spectrophotometry & Beer's Law Video Lecture - Inorganic Chemistry
| 1. What is spectrophotometry? |  |
Ans. Spectrophotometry is a technique used to measure the amount of light absorbed or transmitted by a substance as a function of wavelength. It is commonly used in chemistry, biology, and biochemistry to determine the concentration or purity of a sample.
| 2. What is Beer's Law? | |
Ans. Beer's Law, also known as the Beer-Lambert Law, describes the relationship between the concentration of a solute in a solution and the amount of light absorbed by the solution. It states that the absorbance of a sample is directly proportional to its concentration and the path length of the light through the solution.
| 3. How does spectrophotometry work? | |
Ans. Spectrophotometry works by passing a beam of light through a sample and measuring the intensity of the light that is absorbed or transmitted. The instrument used, called a spectrophotometer, consists of a light source, a monochromator to select a specific wavelength, a sample holder, and a detector. The detector measures the intensity of the light before and after it passes through the sample, allowing the calculation of absorbance or transmittance.
| 4. What are the applications of spectrophotometry? | |
Ans. Spectrophotometry has a wide range of applications in various fields. In chemistry, it is used to determine the concentration of a solute, identify unknown substances, and study reaction kinetics. In biochemistry and molecular biology, it is used for DNA and protein quantification, enzyme activity assays, and drug discovery. It is also used in environmental analysis, pharmaceutical analysis, and quality control in industries such as food and beverage.
| 5. How can Beer's Law be used in spectrophotometry? | |
Ans. Beer's Law is used in spectrophotometry to quantitatively determine the concentration of a solute in a solution. By measuring the absorbance of a sample at a specific wavelength and comparing it to a calibration curve, which relates absorbance to concentration, the unknown concentration of the solute can be determined. This is particularly useful in chemical and biochemical analyses where accurate and precise measurements of concentration are required.
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