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CRE LOX P Recombinase Video Lecture | Crash Course for CSIR NET Life Sciences

FAQs on CRE LOX P Recombinase Video Lecture - Crash Course for CSIR NET Life Sciences

1. What is the role of CRE LOX P Recombinase in site-specific recombination?

Ans.CRE LOX P Recombinase is an enzyme that facilitates site-specific recombination between two specific DNA sequences known as LOX sites. This process allows for precise genetic modifications, such as gene insertion, deletion, or replacement, which is invaluable in genetic engineering and research applications.

2. How do bacteriophages utilize site-specific recombination?

Ans.Bacteriophages, which are viruses that infect bacteria, often employ site-specific recombination to integrate their genetic material into the host bacterial genome. This integration is crucial for the bacteriophage's lifecycle, enabling it to replicate and propagate within bacterial cells.

3. What are the applications of CRE LOX P Recombinase technology in research and medicine?

Ans.CRE LOX P Recombinase technology is widely used in genetic engineering, transgenic animal models, and gene therapy. It allows researchers to create conditional knockouts in mice, study gene functions, and develop targeted therapies for genetic disorders by enabling precise control over gene expression and manipulation.

4. Can you explain the significance of the LOX sites in the CRE LOX system?

Ans.LOX sites are specific DNA sequences recognized by the CRE recombinase. The presence of these sites allows for the targeted recombination events when CRE is expressed. The functionality of the LOX sites ensures that recombination occurs only at desired locations, thereby minimizing unintended genetic changes.

5. What is the importance of understanding site-specific recombination in the context of genetic research?

Ans.Understanding site-specific recombination is crucial in genetic research because it underpins many techniques used for manipulating genomes. This knowledge allows scientists to develop innovative strategies for studying gene functions, creating new models for diseases, and designing targeted therapies, ultimately advancing the field of genetics and biotechnology.

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Sep 28, 2025 Last updated

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