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Paper chromatography Video Lecture | Crash Course for CSIR NET Life Sciences
FAQs on Paper chromatography Video Lecture - Crash Course for CSIR NET Life Sciences
| 1. What is paper chromatography and how does it work? |  |
Ans. Paper chromatography is a technique used to separate and analyze mixtures of substances. It involves placing a small sample of the mixture on a piece of chromatography paper and then allowing a solvent to flow over the paper. As the solvent moves, it carries different components of the mixture at different rates, leading to their separation. The components can then be identified based on their positions on the paper.
| 2. What are the main applications of paper chromatography? | |
Ans. Paper chromatography is widely used in various fields, including biochemistry, food science, and environmental analysis. It is commonly used for the separation of pigments in plants, the analysis of amino acids, and the detection of food additives or contaminants. Additionally, it is a valuable tool in forensic science for analyzing inks and dyes.
| 3. What are the different types of paper chromatography? | |
Ans. The main types of paper chromatography include ascending paper chromatography, descending paper chromatography, and two-dimensional paper chromatography. In ascending chromatography, the solvent moves upward through capillary action; in descending chromatography, it flows downward. Two-dimensional chromatography involves running the solvent in two different directions, allowing for more complex separations.
| 4. What factors affect the separation process in paper chromatography? | |
Ans. Several factors can influence the separation process in paper chromatography, including the choice of solvent, the nature of the paper, the temperature, and the time allowed for the separation. The polarity of the solvent and the components in the mixture also play a critical role in determining how effectively the substances are separated.
| 5. How can the results of paper chromatography be analyzed and interpreted? | |
Ans. The results of paper chromatography can be analyzed by measuring the distance traveled by each component from the baseline and comparing it to the distance traveled by the solvent front. This ratio, known as the Rf value (retention factor), is calculated as Rf = distance traveled by substance / distance traveled by solvent. Rf values can help identify components by comparing them with known standards.
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