Open App

CSIR NET Life Science Exam > CSIR NET Life Science Videos > Crash Course for CSIR NET Life Sciences > Size Exclusion Chromatography

Size Exclusion Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences

FAQs on Size Exclusion Chromatography Video Lecture - Crash Course for CSIR NET Life Sciences

1. What is size exclusion chromatography and how does it work?

Ans.Size exclusion chromatography (SEC), also known as gel filtration or molecular sieve chromatography, is a technique used to separate molecules based on their size. The process involves a column filled with porous beads, where smaller molecules can enter the pores while larger molecules cannot. As a result, larger molecules elute from the column first, followed by smaller ones. This method is commonly used for purifying proteins, polysaccharides, and other macromolecules.

2. What are the main applications of size exclusion chromatography?

Ans.Size exclusion chromatography has several important applications, including the purification of proteins and nucleic acids, desalting of samples, and determining molecular weight. It is widely used in biochemistry, pharmaceuticals, and biotechnology for characterizing biomolecules and assessing their purity.

3. How does the choice of gel affect the results in size exclusion chromatography?

Ans.The choice of gel in size exclusion chromatography is crucial as it determines the separation range and resolution of the method. Different gels have various pore sizes, which can selectively allow certain sizes of molecules to enter. Selecting the appropriate gel based on the size of the target molecules ensures optimal separation and improves the accuracy of results.

4. What are the limitations of size exclusion chromatography?

Ans.Size exclusion chromatography has some limitations, including its inability to separate molecules of similar sizes effectively and the potential for sample loss due to adsorption onto the gel. Additionally, it generally has lower resolution compared to other chromatographic techniques. The method is also not suitable for separating highly polydisperse samples or those with very large size differences.

5. How does size exclusion chromatography compare to other chromatographic techniques?

Ans.Size exclusion chromatography differs from other techniques such as affinity chromatography and ion-exchange chromatography, which separate molecules based on specific interactions rather than size. While SEC is primarily size-based, affinity chromatography targets specific binding interactions between molecules, and ion-exchange chromatography separates based on charge. Each technique has its advantages and ideal applications, often complementing each other in complex separations.

About this Video

Sep 28, 2025 Last updated

Related Exams

UGC NET

Video Description: Size Exclusion Chromatography for CSIR NET Life Science 2025 is part of Crash Course for CSIR NET Life Sciences preparation. The notes and questions for Size Exclusion Chromatography have been prepared according to the CSIR NET Life Science exam syllabus. Information about Size Exclusion Chromatography covers all important topics for CSIR NET Life Science 2025 Exam. Find important definitions, questions, notes, meanings, examples, exercises and tests below for Size Exclusion Chromatography.

Introduction of Size Exclusion Chromatography in English is available as part of our Crash Course for CSIR NET Life Sciences for CSIR NET Life Science & Size Exclusion Chromatography in Hindi for Crash Course for CSIR NET Life Sciences course. Download more important topics related with notes, lectures and mock test series for CSIR NET Life Science Exam by signing up for free.

Description

Video Lecture & Questions for Size Exclusion Chromatography Video Lecture | Crash Course for CSIR NET Life Sciences - CSIR NET Life Science full syllabus preparation | Free video for CSIR NET Life Science exam to prepare for Crash Course for CSIR NET Life Sciences.

Information about Size Exclusion Chromatography

Here you can find the meaning of Size Exclusion Chromatography defined & explained in the simplest way possible. Besides explaining types of Size Exclusion Chromatography theory, EduRev gives you an ample number of questions to practice Size Exclusion Chromatography tests, examples and also practice CSIR NET Life Science tests.

	Crash Course for CSIR NET Life Sciences 256 videos

Crash Course for CSIR NET Life Sciences

256 videos

Join Course for Free

Objective type Questions