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Flow Cytometry Analysis Video Lecture | Crash Course for CSIR NET Life Sciences

FAQs on Flow Cytometry Analysis Video Lecture - Crash Course for CSIR NET Life Sciences

1. What is flow cytometry and how does it work?

Ans. Flow cytometry is a technique used to analyze the physical and chemical characteristics of cells or particles in a fluid as they pass through a laser. It works by passing a stream of cells through a laser beam, where light is scattered and detected. The scattered light provides information about the size, granularity, and fluorescence of the cells, allowing for the identification and quantification of different cell populations.

2. What are the main applications of flow cytometry in biological research?

Ans. Flow cytometry has a wide range of applications in biological research, including immunology for analyzing immune cell populations, cancer research for identifying and characterizing tumor cells, cell cycle analysis to study cell proliferation, and stem cell research to assess differentiation. It is also used in clinical diagnostics for monitoring diseases and in drug discovery to evaluate the effects of compounds on cellular functions.

3. What types of fluorescent dyes are commonly used in flow cytometry?

Ans. Common fluorescent dyes used in flow cytometry include fluorescein isothiocyanate (FITC), phycoerythrin (PE), allophycocyanin (APC), and peridinin-chlorophyll protein (PerCP). These dyes are chosen based on their excitation and emission spectra, allowing for multiplexing where multiple parameters can be analyzed simultaneously by using different colors.

4. How can flow cytometry contribute to the field of clinical diagnostics?

Ans. Flow cytometry contributes to clinical diagnostics by providing rapid and accurate analysis of cell populations in blood and other body fluids. It is commonly used for diagnosing hematological malignancies, such as leukemia and lymphoma, by identifying abnormal cell populations. Additionally, it helps in monitoring immune responses in various diseases and assessing the efficacy of therapies.

5. What are the key considerations for preparing samples for flow cytometry analysis?

Ans. Key considerations for preparing samples for flow cytometry include ensuring that the cells are in a single-cell suspension to avoid clumping, selecting appropriate fluorescent markers for the desired analysis, and optimizing the staining protocol to minimize background fluorescence. Additionally, proper controls, such as unstained and single-stained samples, should be included to ensure accurate interpretation of the data.

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Sep 28, 2025 Last updated

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