DNA Polymerase - 2 Video Lecture | Crash Course for CSIR NET Life Sciences (English)

FAQs on DNA Polymerase - 2 Video Lecture - Crash Course for CSIR NET Life Sciences (English)

1. What is the role of DNA Polymerase in DNA replication?
Ans.DNA Polymerase is an essential enzyme that synthesizes new strands of DNA during the process of DNA replication. It adds nucleotides to the growing DNA chain complementary to the template strand, ensuring accurate duplication of the genetic material for cell division.
2. How does DNA Polymerase ensure accuracy during DNA synthesis?
Ans.DNA Polymerase has a proofreading ability that allows it to detect and correct errors during DNA synthesis. It checks the newly added nucleotide against the template strand and can remove incorrectly paired nucleotides using its exonuclease activity, thereby maintaining the fidelity of DNA replication.
3. What are the different types of DNA Polymerases, and what are their functions?
Ans.There are several types of DNA Polymerases, each with specific roles. For instance, DNA Polymerase I is involved in removing RNA primers and replacing them with DNA during replication. DNA Polymerase III is the main enzyme responsible for DNA synthesis in prokaryotes, while other eukaryotic DNA Polymerases (like α, δ, and ε) serve various functions in replication, repair, and mitochondrial DNA synthesis.
4. What factors can affect the activity of DNA Polymerases?
Ans.Factors such as temperature, pH, the concentration of nucleotides, and the presence of metal ions (like Mg²⁺) can significantly influence the activity of DNA Polymerases. Optimal conditions are necessary for the enzyme to function efficiently, as deviations can lead to reduced activity or denaturation.
5. Why is DNA Polymerase important in biotechnology and molecular biology research?
Ans.DNA Polymerase is crucial in biotechnology, particularly in techniques such as Polymerase Chain Reaction (PCR), where it is used to amplify specific DNA sequences. Its ability to synthesize DNA accurately and rapidly makes it a vital tool for cloning, sequencing, and analyzing genetic material in research and diagnostic applications.
Related Searches

DNA Polymerase - 2 Video Lecture | Crash Course for CSIR NET Life Sciences (English)

,

Sample Paper

,

Viva Questions

,

MCQs

,

study material

,

Previous Year Questions with Solutions

,

Important questions

,

Summary

,

practice quizzes

,

mock tests for examination

,

Free

,

Extra Questions

,

past year papers

,

Exam

,

video lectures

,

shortcuts and tricks

,

Semester Notes

,

Objective type Questions

,

ppt

,

pdf

,

DNA Polymerase - 2 Video Lecture | Crash Course for CSIR NET Life Sciences (English)

,

DNA Polymerase - 2 Video Lecture | Crash Course for CSIR NET Life Sciences (English)

;