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To prepare a temporary stained mount of a leaf peel to show stomata - Lab Experiment Video Lecture - Class 10

FAQs on To prepare a temporary stained mount of a leaf peel to show stomata - Lab Experiment Video Lecture - Class 10

1. What is a stained mount of a leaf peel?
Ans. A stained mount of a leaf peel refers to a microscope slide prepared by staining a thin section of a leaf with a specific stain, in order to highlight the stomata present on the leaf surface. Stomata are tiny openings present on the epidermis of leaves that allow for gas exchange.
2. How can I prepare a temporary stained mount of a leaf peel to show stomata?
Ans. To prepare a temporary stained mount of a leaf peel, follow these steps: 1. Take a fresh leaf and gently remove a small section of its epidermis using forceps. 2. Place the leaf peel on a clean microscope slide. 3. Add a few drops of a suitable stain, such as safranin or iodine solution, on the leaf peel. 4. Gently place a coverslip over the leaf peel, ensuring there are no air bubbles. 5. Carefully observe the slide under a microscope, adjusting the focus to view the stomata.
3. Why is staining necessary in the preparation of a leaf peel mount?
Ans. Staining is necessary in the preparation of a leaf peel mount to make the stomata more visible and distinguishable. The stain helps to enhance the contrast between the stomata and the surrounding leaf tissues, making it easier to observe and study them under a microscope.
4. What are stomata and why are they important?
Ans. Stomata are small openings or pores present on the epidermis of leaves. They play a crucial role in the process of photosynthesis and respiration in plants. Stomata allow for the exchange of gases, such as oxygen and carbon dioxide, between the plant and its surroundings. They also regulate the loss of water vapor through transpiration, helping to maintain the plant's water balance.
5. Can I use any stain for preparing a leaf peel mount to show stomata?
Ans. No, not all stains are suitable for preparing a leaf peel mount to show stomata. It is important to use specific stains that can highlight the stomata effectively. Some commonly used stains for this purpose include safranin, iodine solution, or eosin. These stains help to differentiate the stomata from the surrounding leaf tissues, allowing for better observation and study under a microscope.
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