Anuradha Puri

EduRev CA CPT

Anuradha Puri
EduRev CA CPT
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Discussed Questions
Rahul Dps asked   •  Dec 05, 2019

Calculate number of peptide(s) formed after treatment of following polypeptide with Chemotrypsin.:
Lys-Phe-Gly-Asp-Trp-Ser-Arg-Trp-Leu-His
    Correct answer is '4'. Can you explain this answer?

    Sinjini Singh answered
    Calculation of Peptides Formed After Chemotrypsin Treatment of Polypeptide

    Chemotrypsin is a proteolytic enzyme that cleaves peptide bonds after the amino acid residues like phenylalanine, tryptophan, and tyrosine. When the polypeptide Lys-Phe-Gly-Asp-Trp-Ser-Arg-Trp-Leu-His is treated with chemotrypsin, it undergoes hydrolysis to form several peptides. The calculation of the num
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    Rahul Dps asked   •  Jan 11, 2019

    The J = 0 to J = 1 rotational transition for 1H79Br occurs at 500.72 GHz. Assuming the molecule to be a rigid rotor, the J = 3 to J = 4 transition occurs at …….(in cm–1)
      Correct answer is between '66,67'. Can you explain this answer?

      550.79 GHz.

      This can be calculated using the formula for the frequency of a rotational transition:

      ν = (2B(J+1) - 2BJ) / c

      where ν is the frequency of the transition, B is the rotational constant, J is the initial rotational quantum number, and c is the speed of light.

      Solving for B and plugging in the given values for ν and J, we get:

      B = νc
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