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Page 1 Molecular Phylogeny- Introduction 0 Subject: Bioinformatics Lesson: Molecular Phylogeny- Introduction Lesson Developer: Shailendra Goel College/ Department: Department of Botany, University of Delhi Page 2 Molecular Phylogeny- Introduction 0 Subject: Bioinformatics Lesson: Molecular Phylogeny- Introduction Lesson Developer: Shailendra Goel College/ Department: Department of Botany, University of Delhi Molecular Phylogeny- Introduction 1 Table of Contents Chapter: Molecular Phylogeny Introduction How to generate trees Positive and negative selection Understanding Trees Cladograms vs Phylograms Rooted vs Unrooted trees Tree Terminology Methods of Phylogenetic reconstruction Distance Method UPGMA distance based method Neighbor joining method Statistical methods of phylogeny ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Page 3 Molecular Phylogeny- Introduction 0 Subject: Bioinformatics Lesson: Molecular Phylogeny- Introduction Lesson Developer: Shailendra Goel College/ Department: Department of Botany, University of Delhi Molecular Phylogeny- Introduction 1 Table of Contents Chapter: Molecular Phylogeny Introduction How to generate trees Positive and negative selection Understanding Trees Cladograms vs Phylograms Rooted vs Unrooted trees Tree Terminology Methods of Phylogenetic reconstruction Distance Method UPGMA distance based method Neighbor joining method Statistical methods of phylogeny ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Molecular Phylogeny- Introduction 2 Introduction Mutation is the basis of evolution driven by the process of selection. All life forms are expected to be part of a tree of life, which should be able to explain their origin and evolution. Practically, this may not happen due to extinction of species and further complications arising from ways by which organisms can acquire genes (e.g. lateral transfer of genes). Phylogenetics exploits available comparative information to generate trees, which can explain evolution. Traditionally morphological features were used to compare data and generate trees. More recently molecular sequences are used for comparisons among species, helping in defining species, families and other taxa, hence named as “Molecular Phylogeny”. How to generate trees Trees are generated by comparing traits among organisms. For classical phylogeny these traits are morphological traits but for molecular phylogeny we can use DNA, RNA or protein sequence data. As a general rule DNA has more phylogenetic information as compared to proteins. Proteins are derived through triplet code, in which third bases follow the “wobble hypothesis” leading to loss of phylogenetic information. DNA sequences comprise coding and non-coding regions that have differing rates of evolution. The rate of evolution also depends on the type of organism. Comparison of sequences can only be done after aligning them. Without alignment it is very difficult to decide which nucleotide/amino acid should be compared with which one (homology). Proteins show two types of changes- synonymous and non-synonymous. A synonymous change does not result in change in the coded amino acid. Positive and negative selection Traditionally, any change which is favored by natural selection is called positive selection. It is favored by natural selection because it helps in the survival of organism. Similarly, any trait which is not favored by natural selection is normally eliminated and is called negative selection. Page 4 Molecular Phylogeny- Introduction 0 Subject: Bioinformatics Lesson: Molecular Phylogeny- Introduction Lesson Developer: Shailendra Goel College/ Department: Department of Botany, University of Delhi Molecular Phylogeny- Introduction 1 Table of Contents Chapter: Molecular Phylogeny Introduction How to generate trees Positive and negative selection Understanding Trees Cladograms vs Phylograms Rooted vs Unrooted trees Tree Terminology Methods of Phylogenetic reconstruction Distance Method UPGMA distance based method Neighbor joining method Statistical methods of phylogeny ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Molecular Phylogeny- Introduction 2 Introduction Mutation is the basis of evolution driven by the process of selection. All life forms are expected to be part of a tree of life, which should be able to explain their origin and evolution. Practically, this may not happen due to extinction of species and further complications arising from ways by which organisms can acquire genes (e.g. lateral transfer of genes). Phylogenetics exploits available comparative information to generate trees, which can explain evolution. Traditionally morphological features were used to compare data and generate trees. More recently molecular sequences are used for comparisons among species, helping in defining species, families and other taxa, hence named as “Molecular Phylogeny”. How to generate trees Trees are generated by comparing traits among organisms. For classical phylogeny these traits are morphological traits but for molecular phylogeny we can use DNA, RNA or protein sequence data. As a general rule DNA has more phylogenetic information as compared to proteins. Proteins are derived through triplet code, in which third bases follow the “wobble hypothesis” leading to loss of phylogenetic information. DNA sequences comprise coding and non-coding regions that have differing rates of evolution. The rate of evolution also depends on the type of organism. Comparison of sequences can only be done after aligning them. Without alignment it is very difficult to decide which nucleotide/amino acid should be compared with which one (homology). Proteins show two types of changes- synonymous and non-synonymous. A synonymous change does not result in change in the coded amino acid. Positive and negative selection Traditionally, any change which is favored by natural selection is called positive selection. It is favored by natural selection because it helps in the survival of organism. Similarly, any trait which is not favored by natural selection is normally eliminated and is called negative selection. Molecular Phylogeny- Introduction 3 Similar kind of selection also operates for molecular sequences. It is common among genes to go through duplication. A duplicated copy of gene is free to undergo mutation and create variation. This variation goes through positive/negative selection and often leads to neofunctionalization, leading to new genes with new functions. Understanding Trees Cladograms vs Phylograms Trees fall under two categories – Cladogram and Phylogram. Cladogram just provide the information about relationship between different organisms while phylograms also provide a measure of the amount of evolutionary change, as seen in the branch-lengths. Due to this fact, branch length has no meaning in cladograms while it has meaning in phylograms. Figure: Phylogram Figure: Cladogram Source: Author Source: Author Rooted vs Unrooted trees The root in a tree denotes the ultimate common ancestor and provides direction in time. At times, it is not possible to have this information hence there are both types of algorithms available- those we do apply a common ancestor hypothesis and those we does not. A Page 5 Molecular Phylogeny- Introduction 0 Subject: Bioinformatics Lesson: Molecular Phylogeny- Introduction Lesson Developer: Shailendra Goel College/ Department: Department of Botany, University of Delhi Molecular Phylogeny- Introduction 1 Table of Contents Chapter: Molecular Phylogeny Introduction How to generate trees Positive and negative selection Understanding Trees Cladograms vs Phylograms Rooted vs Unrooted trees Tree Terminology Methods of Phylogenetic reconstruction Distance Method UPGMA distance based method Neighbor joining method Statistical methods of phylogeny ? Summary ? Exercise/ Practice ? Glossary ? References/ Bibliography/ Further Reading Molecular Phylogeny- Introduction 2 Introduction Mutation is the basis of evolution driven by the process of selection. All life forms are expected to be part of a tree of life, which should be able to explain their origin and evolution. Practically, this may not happen due to extinction of species and further complications arising from ways by which organisms can acquire genes (e.g. lateral transfer of genes). Phylogenetics exploits available comparative information to generate trees, which can explain evolution. Traditionally morphological features were used to compare data and generate trees. More recently molecular sequences are used for comparisons among species, helping in defining species, families and other taxa, hence named as “Molecular Phylogeny”. How to generate trees Trees are generated by comparing traits among organisms. For classical phylogeny these traits are morphological traits but for molecular phylogeny we can use DNA, RNA or protein sequence data. As a general rule DNA has more phylogenetic information as compared to proteins. Proteins are derived through triplet code, in which third bases follow the “wobble hypothesis” leading to loss of phylogenetic information. DNA sequences comprise coding and non-coding regions that have differing rates of evolution. The rate of evolution also depends on the type of organism. Comparison of sequences can only be done after aligning them. Without alignment it is very difficult to decide which nucleotide/amino acid should be compared with which one (homology). Proteins show two types of changes- synonymous and non-synonymous. A synonymous change does not result in change in the coded amino acid. Positive and negative selection Traditionally, any change which is favored by natural selection is called positive selection. It is favored by natural selection because it helps in the survival of organism. Similarly, any trait which is not favored by natural selection is normally eliminated and is called negative selection. Molecular Phylogeny- Introduction 3 Similar kind of selection also operates for molecular sequences. It is common among genes to go through duplication. A duplicated copy of gene is free to undergo mutation and create variation. This variation goes through positive/negative selection and often leads to neofunctionalization, leading to new genes with new functions. Understanding Trees Cladograms vs Phylograms Trees fall under two categories – Cladogram and Phylogram. Cladogram just provide the information about relationship between different organisms while phylograms also provide a measure of the amount of evolutionary change, as seen in the branch-lengths. Due to this fact, branch length has no meaning in cladograms while it has meaning in phylograms. Figure: Phylogram Figure: Cladogram Source: Author Source: Author Rooted vs Unrooted trees The root in a tree denotes the ultimate common ancestor and provides direction in time. At times, it is not possible to have this information hence there are both types of algorithms available- those we do apply a common ancestor hypothesis and those we does not. A Molecular Phylogeny- Introduction 4 common way to decide the root of tree is by using an outgroup. An outgroup is a taxon from a group closely related to the ingroup, which includes the taxa under study. Another way to identify the root is to use midpoint as the rooting point for the longest branch. Figure: Midpoint Rooted Tree Figure: Outgroup rooted tree Source: Author Source: Author Tree Terminology Trees can be described based on branches and nodes. Terminal branches represent Operational Taxonomic Unites (OTU’s). When two branches are connected, it results in internal nodes. When two terminal branches are directly connected to each other, they are called sister branches.Read More
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1. What is molecular phylogeny in botany? |
2. How is molecular phylogeny different from traditional methods of studying plant evolution? |
3. What are some of the benefits of using molecular phylogeny in botany research? |
4. What techniques are commonly used in molecular phylogeny studies? |
5. How does molecular phylogeny contribute to our understanding of plant evolution and conservation? |
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