IIT JAM Exam > IIT JAM Questions > The topological features of circular DNA may ...
Start Learning for Free
The topological features of circular DNA may not affect which of the following?
- a)The electrophoretic mobility of the DNA.
- b)The sedimentation properties of the DNA.
- c)The affinity toward proteins that bind to the DNA.
- d)The susceptibility of the DNA to the action of DNA ligas
Correct answer is option 'B'. Can you explain this answer?
| FREE This question is part of | Download PDF Attempt this Test |
Verified Answer
The topological features of circular DNA may not affect which of the f...
The topological features of DNA can affect all except sedimentation. However, if ethidium bromide (EtBr) is added to different topological forms, it may lead to a change.
Most Upvoted Answer
The topological features of circular DNA may not affect which of the f...
Understanding Circular DNA Topology
Circular DNA, such as plasmids, exhibits unique topological features like supercoiling, which can influence various biochemical properties. However, its effect on sedimentation properties is different from other characteristics.
Electrophoretic Mobility
- The shape and topology of DNA significantly affect its movement in an electric field.
- Supercoiled DNA migrates faster than relaxed DNA due to its compact structure, influencing electrophoretic mobility.
Affinity Toward Proteins
- DNA topology can alter how proteins, such as transcription factors or restriction enzymes, interact with the DNA.
- The binding affinity may change based on the supercoiling state, which can expose or obscure binding sites.
Sedimentation Properties
- Sedimentation involves the separation of molecules based on their size and mass in a centrifugal field.
- Circular DNA, irrespective of its topology (supercoiled or relaxed), has similar sedimentation coefficients due to its uniform shape.
- This means that the sedimentation rate is primarily determined by the overall size and density rather than the topological state of the DNA.
Susceptibility to DNA Ligase
- The action of DNA ligase on circular DNA can be influenced by topology, but generally, ligase can act on both supercoiled and relaxed forms.
- The presence of nicks or breaks can be addressed regardless of the DNA's topological state.
Conclusion
In summary, while the topological features of circular DNA affect electrophoretic mobility, protein binding affinity, and ligase activity, they do not significantly impact sedimentation properties, making option 'B' the correct choice.
Circular DNA, such as plasmids, exhibits unique topological features like supercoiling, which can influence various biochemical properties. However, its effect on sedimentation properties is different from other characteristics.
Electrophoretic Mobility
- The shape and topology of DNA significantly affect its movement in an electric field.
- Supercoiled DNA migrates faster than relaxed DNA due to its compact structure, influencing electrophoretic mobility.
Affinity Toward Proteins
- DNA topology can alter how proteins, such as transcription factors or restriction enzymes, interact with the DNA.
- The binding affinity may change based on the supercoiling state, which can expose or obscure binding sites.
Sedimentation Properties
- Sedimentation involves the separation of molecules based on their size and mass in a centrifugal field.
- Circular DNA, irrespective of its topology (supercoiled or relaxed), has similar sedimentation coefficients due to its uniform shape.
- This means that the sedimentation rate is primarily determined by the overall size and density rather than the topological state of the DNA.
Susceptibility to DNA Ligase
- The action of DNA ligase on circular DNA can be influenced by topology, but generally, ligase can act on both supercoiled and relaxed forms.
- The presence of nicks or breaks can be addressed regardless of the DNA's topological state.
Conclusion
In summary, while the topological features of circular DNA affect electrophoretic mobility, protein binding affinity, and ligase activity, they do not significantly impact sedimentation properties, making option 'B' the correct choice.
|
Explore Courses for IIT JAM exam
|
|
Similar IIT JAM Doubts
The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer?
Question Description
The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer?.
The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer?.
Solutions for The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM.
Download more important topics, notes, lectures and mock test series for IIT JAM Exam by signing up for free.
Here you can find the meaning of The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? defined & explained in the simplest way possible. Besides giving the explanation of
The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer?, a detailed solution for The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? has been provided alongside types of The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice The topological features of circular DNA may not affect which of the following?a)The electrophoretic mobility of the DNA.b)The sedimentation properties of the DNA.c)The affinity toward proteins that bind to the DNA.d)The susceptibility of the DNA to the action of DNA ligasCorrect answer is option 'B'. Can you explain this answer? tests, examples and also practice IIT JAM tests.
|
|
Explore Courses for IIT JAM exam
|
|
Suggested Free Tests
Signup for Free!
Signup to see your scores go up within 7 days! Learn & Practice with 1000+ FREE Notes, Videos & Tests.
|
© EduRev
|
Education Revolution
|
|
Signup to see your scores
go up within 7 days!
Access 1000+ FREE Docs, Videos and Tests
Takes less than 10 seconds to signup