Which of the following statements is not correct about gel electrophor...
Electric field is dependent inversely on length of gel, it is related by the expression E = v/l, Where E is electric field, v is applied voltage and l is length of gel or distance between electrodes.
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Which of the following statements is not correct about gel electrophor...
Gel Electrophoresis
Gel electrophoresis is a technique used to separate biomolecules based on their size, shape, and charge.
Electrophoretic Mobility
The electrophoretic mobility of biomolecules is affected by their shape and size. This means that different molecules will move at different rates through the gel, allowing for their separation.
Electric Field
The electric field (E) is not affected by the length of the gel. This statement is incorrect. The electric field is directly proportional to the length of the gel.
Velocity
The velocity of a molecule in electrophoretic gel is inversely related to the frictional drag encountered in the matrix of the gel. This means that molecules that encounter more friction will move more slowly through the gel, while those that encounter less friction will move more quickly.
Polyacrylamide Gel
Polyacrylamide gel can be used for nucleic acids separation. This statement is correct. Polyacrylamide gel is a commonly used medium for separating nucleic acids based on their size.
Conclusion
In summary, gel electrophoresis is a powerful technique used to separate biomolecules based on their size, shape, and charge. The length of the gel affects the electric field, and the velocity of a molecule in the gel is inversely related to the frictional drag encountered. Polyacrylamide gel can be used for nucleic acids separation.