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In polyacrylamide gel electrophoresis, SDS is used for the mixture of proteins for their efficient separation on the gel. Why SDS, in this experiment is used?
  • a)
    To stabilize protein.   
  • b)
    To solubilize protein.
  • c)
    To maintain its structure.   
  • d)
    To provide uniform charge density on protein.
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
In polyacrylamide gel electrophoresis, SDS is used for the mixture of ...
Most of the proteins bind SDS with constant-weight ratio (one SDS molecule per two amino acids) leading to identical charge densities. However, unless proteins are completely unfolded, SDS cannot bind uniformly to protein amino acid residues as some amino acid residues may not be accessible due to folding of protein. Thus reducing agent further denatures the proteins by reducing disulfide linkages and enables SDS to binds the proteins uniformily.
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In polyacrylamide gel electrophoresis, SDS is used for the mixture of proteins for their efficient separation on the gel. Why SDS, in this experiment is used?a)To stabilize protein.b)To solubilize protein.c)To maintain its structure.d)To provide uniform charge density on protein.Correct answer is option 'D'. Can you explain this answer?
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In polyacrylamide gel electrophoresis, SDS is used for the mixture of proteins for their efficient separation on the gel. Why SDS, in this experiment is used?a)To stabilize protein.b)To solubilize protein.c)To maintain its structure.d)To provide uniform charge density on protein.Correct answer is option 'D'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about In polyacrylamide gel electrophoresis, SDS is used for the mixture of proteins for their efficient separation on the gel. Why SDS, in this experiment is used?a)To stabilize protein.b)To solubilize protein.c)To maintain its structure.d)To provide uniform charge density on protein.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In polyacrylamide gel electrophoresis, SDS is used for the mixture of proteins for their efficient separation on the gel. Why SDS, in this experiment is used?a)To stabilize protein.b)To solubilize protein.c)To maintain its structure.d)To provide uniform charge density on protein.Correct answer is option 'D'. Can you explain this answer?.
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