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A scientist is working for the discovery of a biomarker for a particular disease. Find out the right options which he will used for this discovery.
  • a)
    For the discovery phase he will use western BLOT or ELISA
  • b)
    For the discovery phase he will use 2-D electrophoresis
  • c)
    For the validating phase he will use western BLOT or ELISA
  • d)
    For the validating phase he will use 2-D gel electrophoresis
Correct answer is option 'B,C'. Can you explain this answer?
Verified Answer
A scientist is working for the discovery of a biomarker for a particul...
2-D gel electrophoresis is used for the discovery phase in proteomics approach, whatever the proteins have been identified by 2-D gel electrophoresis then these are validates by either ELISA or western blot.
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Explanation:The separation techniques of proteins are used to isolate and purify proteins from complex mixtures. These techniques are based on various properties of proteins such as solubility, charge, size, and others. However, the technique of protein separation based on viscosity is not commonly used. Protein solubility:Protein solubility refers to the ability of a protein to dissolve in a given solvent. Different proteins have different solubilities in different solvents. This property is often used in techniques like precipitation, where proteins are selectively precipitated out of solution based on their solubility.Protein charge:Protein charge refers to the net charge of a protein molecule, which is determined by the presence of charged amino acid residues such as lysine, arginine, aspartic acid, and glutamic acid. Techniques like electrophoresis and ion exchange chromatography utilize the charge differences between proteins to separate them.Protein size:Protein size refers to the molecular weight or size of a protein molecule. Techniques such as gel filtration chromatography and SDS-PAGE separate proteins based on their size. In gel filtration chromatography, proteins are separated based on their ability to enter and pass through a porous gel matrix, while in SDS-PAGE, proteins are separated based on their migration through a gel under the influence of an electric field.Protein viscosity:Protein viscosity refers to the resistance of a protein solution to flow. While protein viscosity can vary depending on factors such as protein concentration, temperature, and pH, it is not typically used as a property for protein separation. In conclusion, the separation techniques of proteins are based on properties such as solubility, charge, and size. Protein viscosity is not commonly used as a property for protein separation.

A scientist is working for the discovery of a biomarker for a particular disease. Find out the right options which he will used for this discovery.a)For the discovery phase he will use western BLOT or ELISAb)For the discovery phase he will use 2-D electrophoresisc)For the validating phase he will use western BLOT or ELISAd)For the validating phase he will use 2-D gel electrophoresisCorrect answer is option 'B,C'. Can you explain this answer?
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A scientist is working for the discovery of a biomarker for a particular disease. Find out the right options which he will used for this discovery.a)For the discovery phase he will use western BLOT or ELISAb)For the discovery phase he will use 2-D electrophoresisc)For the validating phase he will use western BLOT or ELISAd)For the validating phase he will use 2-D gel electrophoresisCorrect answer is option 'B,C'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A scientist is working for the discovery of a biomarker for a particular disease. Find out the right options which he will used for this discovery.a)For the discovery phase he will use western BLOT or ELISAb)For the discovery phase he will use 2-D electrophoresisc)For the validating phase he will use western BLOT or ELISAd)For the validating phase he will use 2-D gel electrophoresisCorrect answer is option 'B,C'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A scientist is working for the discovery of a biomarker for a particular disease. Find out the right options which he will used for this discovery.a)For the discovery phase he will use western BLOT or ELISAb)For the discovery phase he will use 2-D electrophoresisc)For the validating phase he will use western BLOT or ELISAd)For the validating phase he will use 2-D gel electrophoresisCorrect answer is option 'B,C'. Can you explain this answer?.
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