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DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer is
- a)Phosphatidyl ethanolamine
- b)EDTA is a chelating ligand hence its sequester the divalent ion
- c)DNase enzymes, which cleave DNA are known to depend upon Mg2+ ions.
- d)EDTA interacts with negative phosphate groups of DNA and protect from degradation
Correct answer is option 'B,C'. Can you explain this answer?
Verified Answer
DNA can be stored in TE buffer containing tris and EDTA, The primary r...
DNase are known to depend upon the divalent ions such as magnesium ions, which can be sequestered (blocked) in solution by addition of chelating agents such as EDTA. Hence, EDTA is added to DNA storage solution from preventing it from DNase mediated degradation.
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DNA can be stored in TE buffer containing tris and EDTA, The primary r...
Introduction:
TE buffer is commonly used for the storage and preservation of DNA samples. It consists of Tris (a buffering agent) and EDTA (a chelating agent). The primary reason for adding EDTA to the DNA storing buffer is to protect the DNA from degradation and to prevent the activity of DNase enzymes.
Explanation:
1. Chelating Ligand:
- EDTA is a chelating agent, meaning it has the ability to bind and sequester metal ions.
- In the case of DNA storage, EDTA acts as a chelating ligand by binding to divalent metal ions, particularly magnesium ions (Mg2+), which are required for the activity of DNase enzymes.
- By sequestering the Mg2+ ions, EDTA prevents the DNase enzymes from functioning properly.
2. Protection from DNase enzymes:
- DNase enzymes are responsible for the cleavage of DNA molecules, leading to degradation.
- These enzymes require divalent metal ions like Mg2+ as cofactors for their enzymatic activity.
- By chelating Mg2+ ions, EDTA inhibits the activity of DNase enzymes and protects the DNA from cleavage and degradation.
3. Interaction with DNA:
- EDTA also interacts with the negatively charged phosphate groups present in the DNA backbone.
- The phosphate groups are responsible for the overall negative charge of the DNA molecule.
- EDTA forms stable complexes with the phosphate groups, protecting them from enzymatic cleavage and degradation.
Conclusion:
In conclusion, the primary reasons for adding EDTA to the TE buffer for DNA storage are its ability to chelate divalent metal ions (such as Mg2+), which are required for the activity of DNase enzymes, and its interaction with the negatively charged phosphate groups of DNA, protecting it from degradation. This ensures the preservation and stability of the DNA samples in the storage buffer.
TE buffer is commonly used for the storage and preservation of DNA samples. It consists of Tris (a buffering agent) and EDTA (a chelating agent). The primary reason for adding EDTA to the DNA storing buffer is to protect the DNA from degradation and to prevent the activity of DNase enzymes.
Explanation:
1. Chelating Ligand:
- EDTA is a chelating agent, meaning it has the ability to bind and sequester metal ions.
- In the case of DNA storage, EDTA acts as a chelating ligand by binding to divalent metal ions, particularly magnesium ions (Mg2+), which are required for the activity of DNase enzymes.
- By sequestering the Mg2+ ions, EDTA prevents the DNase enzymes from functioning properly.
2. Protection from DNase enzymes:
- DNase enzymes are responsible for the cleavage of DNA molecules, leading to degradation.
- These enzymes require divalent metal ions like Mg2+ as cofactors for their enzymatic activity.
- By chelating Mg2+ ions, EDTA inhibits the activity of DNase enzymes and protects the DNA from cleavage and degradation.
3. Interaction with DNA:
- EDTA also interacts with the negatively charged phosphate groups present in the DNA backbone.
- The phosphate groups are responsible for the overall negative charge of the DNA molecule.
- EDTA forms stable complexes with the phosphate groups, protecting them from enzymatic cleavage and degradation.
Conclusion:
In conclusion, the primary reasons for adding EDTA to the TE buffer for DNA storage are its ability to chelate divalent metal ions (such as Mg2+), which are required for the activity of DNase enzymes, and its interaction with the negatively charged phosphate groups of DNA, protecting it from degradation. This ensures the preservation and stability of the DNA samples in the storage buffer.
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DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer?
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DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer? for IIT JAM 2025 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer? covers all topics & solutions for IIT JAM 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer?.
DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer? for IIT JAM 2025 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer? covers all topics & solutions for IIT JAM 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer?.
Solutions for DNA can be stored in TE buffer containing tris and EDTA, The primary reason for adding EDTA to DNA storing buffer isa)Phosphatidyl ethanolamineb)EDTA is a chelating ligand hence its sequester the divalent ionc)DNase enzymes, which cleave DNA are known to depend upon Mg2+ions.d)EDTA interacts with negative phosphate groups of DNA and protect from degradationCorrect answer is option 'B,C'. Can you explain this answer? in English & in Hindi are available as part of our courses for IIT JAM.
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