Gentamycin is an antibiotic, which is produced bya)Bacillus sp.b)Cepha...
Gentamycin is an antibiotic produced by the bacteria belonging to Micromonospora sp. It inhibits translation by inhibiting 16s rRNA.
Gentamycin is an antibiotic, which is produced bya)Bacillus sp.b)Cepha...
Introduction:
Gentamycin is an antibiotic that is widely used to treat various bacterial infections. It belongs to the aminoglycoside class of antibiotics and is effective against a wide range of gram-negative and gram-positive bacteria.
Production of Gentamycin:
Gentamycin is produced by the bacterium Micromonospora sp. Micromonospora is a genus of bacteria that belongs to the family Micromonosporaceae and the order Actinomycetales. Actinomycetes are a group of bacteria that are known for their ability to produce a variety of bioactive compounds, including antibiotics.
Process of Gentamycin Production:
The production of Gentamycin by Micromonospora involves several steps:
1. Isolation and identification of Micromonospora strains: Micromonospora strains are isolated from natural sources such as soil, water, and plant material. These strains are then identified using various biochemical and molecular techniques.
2. Optimization of growth conditions: The growth conditions for Micromonospora strains are optimized to enhance the production of Gentamycin. This includes factors such as temperature, pH, nutrient composition, and oxygen availability.
3. Fermentation: The selected Micromonospora strain is grown in large-scale fermentation tanks under controlled conditions. The fermentation medium contains nutrients that support the growth and production of Gentamycin.
4. Extraction: After fermentation, the culture broth is harvested and subjected to extraction to isolate the Gentamycin. This involves the use of organic solvents to extract the antibiotic from the fermentation broth.
5. Purification: The crude extract obtained from the extraction step is further purified using various chromatographic techniques. This helps to remove impurities and isolate the pure Gentamycin compound.
6. Formulation: The purified Gentamycin is then formulated into different dosage forms such as injections, ointments, and eye drops, depending on the intended use.
Conclusion:
In conclusion, Gentamycin is produced by the bacterium Micromonospora sp. through a series of steps including isolation, optimization of growth conditions, fermentation, extraction, purification, and formulation. Micromonospora strains have the ability to produce a variety of bioactive compounds, and Gentamycin is one of the antibiotics produced by these bacteria.