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Dideoxy DNA sequencing not exclusively depends on the following.
  • a)
    Termination
  • b)
    ATP
  • c)
    Vector primer
  • d)
    Plasmid vector
Correct answer is option 'B,C,D'. Can you explain this answer?
Verified Answer
Dideoxy DNA sequencing not exclusively depends on the following.a)Term...
Dideoxy sequencing method is also known as chain termination method because after addition of dideoxy nucleotide, the growing chain terminates because of the absence of OH group in dideoxy nucleotide.
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Dideoxy DNA sequencing not exclusively depends on the following.a)Term...
Introduction:
Dideoxy DNA sequencing, also known as Sanger sequencing, is a widely used method for DNA sequencing. It is based on the selective incorporation of dideoxynucleotides (ddNTPs) during DNA synthesis, leading to the termination of DNA strands at specific positions. This method requires a template DNA, a primer, DNA polymerase, and a set of ddNTPs labeled with different fluorescent dyes.

Explanation:
Dideoxy DNA sequencing does not exclusively depend on the following factors:

Termination:
- The key principle of dideoxy DNA sequencing is the incorporation of ddNTPs, which lack a 3' hydroxyl group necessary for further DNA strand elongation.
- These ddNTPs are incorporated randomly during DNA synthesis, leading to premature termination of the growing DNA strand at specific positions.
- The termination occurs at each nucleotide position, resulting in a collection of DNA fragments of varying lengths.

ATP:
- Adenosine triphosphate (ATP) is not directly involved in the dideoxy DNA sequencing method.
- ATP is a nucleotide used as an energy source in various cellular processes, but it is not required for the termination of DNA strands or the sequencing reaction itself.

Vector Primer:
- A vector primer is a short DNA sequence that hybridizes to the template DNA and provides a starting point for DNA synthesis.
- In dideoxy DNA sequencing, a sequencing primer is used instead of a vector primer.
- The sequencing primer is complementary to the sequence immediately upstream of the region to be sequenced.
- It serves as a starting point for DNA synthesis and enables the incorporation of fluorescently labeled ddNTPs.

Plasmid Vector:
- A plasmid vector is a small, circular DNA molecule used to carry foreign DNA fragments in molecular cloning experiments.
- However, in dideoxy DNA sequencing, the plasmid vector is not directly involved.
- The template DNA used for sequencing can be derived from various sources, such as PCR products or plasmid DNA.
- The plasmid vector is only used for cloning purposes and does not play a role in the sequencing reaction itself.

Conclusion:
In summary, dideoxy DNA sequencing does not exclusively depend on ATP, vector primer, or plasmid vector. Instead, it relies on the selective termination of DNA strands through the incorporation of ddNTPs. The sequencing reaction requires a template DNA, a sequencing primer, DNA polymerase, and a set of labeled ddNTPs.
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Dideoxy DNA sequencing not exclusively depends on the following.a)Terminationb)ATPc)Vector primerd)Plasmid vectorCorrect answer is option 'B,C,D'. Can you explain this answer?
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