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After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________
Correct answer is '3'. Can you explain this answer?
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After PCR of 25 cycles the amount of amplification product obtained is...
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After PCR of 25 cycles the amount of amplification product obtained is...
Given information:
- PCR was carried out for 25 cycles.
- The amount of amplification product obtained after 25 cycles is 12 units.
To find:
- The expected amount of amplification product formed after 23 cycles.
Solution:
The amount of amplification product formed after each cycle of PCR is expected to double. So, after 25 cycles, the amount of amplification product obtained is 12 units.
Calculating the initial amount of amplification product:
To find the initial amount of amplification product, we need to calculate the amount of amplification product formed after the first cycle.
Let's assume the initial amount of amplification product (after 0 cycles) is X units.
- After 1 cycle, the amount of amplification product formed is 2X units.
- After 2 cycles, the amount of amplification product formed is 4X units.
- After 3 cycles, the amount of amplification product formed is 8X units.
- After 4 cycles, the amount of amplification product formed is 16X units.
Calculating the amount of amplification product formed after 23 cycles:
- After 4 cycles, the amount of amplification product formed is 16X units.
- After 5 cycles, the amount of amplification product formed is 32X units.
- After 6 cycles, the amount of amplification product formed is 64X units.
- ...
- After 23 cycles, the amount of amplification product formed is (2^19)X units.
Using the given information:
- After 25 cycles, the amount of amplification product obtained is 12 units.
- We can calculate the value of X using the equation (2^19)X = 12.
Calculating X:
(2^19)X = 12
X = 12 / (2^19)
Calculating the amount of amplification product after 23 cycles:
After 23 cycles, the amount of amplification product formed is (2^19)X units.
= (2^19) * (12 / (2^19))
= 12 units
Hence, the expected amount of amplification product formed after 23 cycles is 3 units.
- PCR was carried out for 25 cycles.
- The amount of amplification product obtained after 25 cycles is 12 units.
To find:
- The expected amount of amplification product formed after 23 cycles.
Solution:
The amount of amplification product formed after each cycle of PCR is expected to double. So, after 25 cycles, the amount of amplification product obtained is 12 units.
Calculating the initial amount of amplification product:
To find the initial amount of amplification product, we need to calculate the amount of amplification product formed after the first cycle.
Let's assume the initial amount of amplification product (after 0 cycles) is X units.
- After 1 cycle, the amount of amplification product formed is 2X units.
- After 2 cycles, the amount of amplification product formed is 4X units.
- After 3 cycles, the amount of amplification product formed is 8X units.
- After 4 cycles, the amount of amplification product formed is 16X units.
Calculating the amount of amplification product formed after 23 cycles:
- After 4 cycles, the amount of amplification product formed is 16X units.
- After 5 cycles, the amount of amplification product formed is 32X units.
- After 6 cycles, the amount of amplification product formed is 64X units.
- ...
- After 23 cycles, the amount of amplification product formed is (2^19)X units.
Using the given information:
- After 25 cycles, the amount of amplification product obtained is 12 units.
- We can calculate the value of X using the equation (2^19)X = 12.
Calculating X:
(2^19)X = 12
X = 12 / (2^19)
Calculating the amount of amplification product after 23 cycles:
After 23 cycles, the amount of amplification product formed is (2^19)X units.
= (2^19) * (12 / (2^19))
= 12 units
Hence, the expected amount of amplification product formed after 23 cycles is 3 units.
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After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer?
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After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer?.
After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for After PCR of 25 cycles the amount of amplification product obtained is 12 unit. If PCR was carried out for 23 cycles, the expected amount of the amplification product formed (in unit) will be _________Correct answer is '3'. Can you explain this answer?.
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