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An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out for
- a)2 min.
- b)5 min.
- c)30 min.
- d)60 min.
Correct answer is option 'B'. Can you explain this answer?
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An enzyme follows Michaelis- Menten kinetics for the conversion of a s...
Explanation:
Michaelis-Menten kinetics is a mathematical model that describes the behavior of enzymes that catalyze a single chemical reaction with a single substrate.
The model assumes that the reaction consists of two steps:
1. Formation of an enzyme-substrate complex (ES).
2. Conversion of the ES complex to product (P) and free enzyme (E).
The rate of the reaction depends on the concentration of the substrate, the concentration of the enzyme, and the rate constant for the conversion of the ES complex to product and free enzyme.
The Michaelis-Menten equation is given as:
V = (Vmax [S])/(Km + [S])
Where V is the initial rate of the reaction, Vmax is the maximum rate of the reaction, [S] is the concentration of the substrate, and Km is the Michaelis constant, which is equal to the concentration of the substrate when the reaction rate is half of Vmax.
To calculate Km, we need to determine the initial rate of the reaction at different substrate concentrations.
In this question, we are given that there is no net conversion of the substrate after 30 minutes of incubation. This means that the reaction has reached equilibrium, and we cannot determine the initial rate of the reaction.
However, we are also given that the product can be reliably detected from 2 minutes after the incubation begins. This means that the reaction has not reached equilibrium at 2 minutes, and we can determine the initial rate of the reaction at this time point.
Therefore, the assay should be carried out for 2 minutes to calculate Km.
Option B is the correct answer.
Michaelis-Menten kinetics is a mathematical model that describes the behavior of enzymes that catalyze a single chemical reaction with a single substrate.
The model assumes that the reaction consists of two steps:
1. Formation of an enzyme-substrate complex (ES).
2. Conversion of the ES complex to product (P) and free enzyme (E).
The rate of the reaction depends on the concentration of the substrate, the concentration of the enzyme, and the rate constant for the conversion of the ES complex to product and free enzyme.
The Michaelis-Menten equation is given as:
V = (Vmax [S])/(Km + [S])
Where V is the initial rate of the reaction, Vmax is the maximum rate of the reaction, [S] is the concentration of the substrate, and Km is the Michaelis constant, which is equal to the concentration of the substrate when the reaction rate is half of Vmax.
To calculate Km, we need to determine the initial rate of the reaction at different substrate concentrations.
In this question, we are given that there is no net conversion of the substrate after 30 minutes of incubation. This means that the reaction has reached equilibrium, and we cannot determine the initial rate of the reaction.
However, we are also given that the product can be reliably detected from 2 minutes after the incubation begins. This means that the reaction has not reached equilibrium at 2 minutes, and we can determine the initial rate of the reaction at this time point.
Therefore, the assay should be carried out for 2 minutes to calculate Km.
Option B is the correct answer.
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An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer?
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An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer?.
An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for An enzyme follows Michaelis- Menten kinetics for the conversion of a substrate to a product.Under certain conditions of assay, there is no net conversion of the substrate after 30 min of incubation. The product can be reliably detected from 2 min after the incubation begins. In order to calculate Km, the assay should be carried out fora)2 min.b)5 min.c)30 min.d)60 min.Correct answer is option 'B'. Can you explain this answer?.
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