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After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogram
From the autoradiogram determine the base sequence of the template strand.
  • a)
    3'-TACGT-5'
  • b)
    5'-TGCAT-3'
  • c)
    3'-ACGTT-5'
  • d)
    5'-ATGCA-3'
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
After using the Sanger method tor sequencing DNA (dideoxy nucleotide m...
The Sangers gels are read from bottom to top to obtain a sequence of DNA. however this is the sequence of newly synthesized strand and template is complementary to this. On reading the gel from bottom we get 5 -TGC'AT-3 '. while complement ary to this would be 3’ACGTA5’, which can be written from 5’to 3’ as 5’ATGCA3’.
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After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogramFrom the autoradiogram determine the base sequence of the template strand.a)3-TACGT-5b)5-TGCAT-3c)3-ACGTT-5d)5-ATGCA-3Correct answer is option 'D'. Can you explain this answer?
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After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogramFrom the autoradiogram determine the base sequence of the template strand.a)3-TACGT-5b)5-TGCAT-3c)3-ACGTT-5d)5-ATGCA-3Correct answer is option 'D'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogramFrom the autoradiogram determine the base sequence of the template strand.a)3-TACGT-5b)5-TGCAT-3c)3-ACGTT-5d)5-ATGCA-3Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for After using the Sanger method tor sequencing DNA (dideoxy nucleotide method), you observe the following autoradiogramFrom the autoradiogram determine the base sequence of the template strand.a)3-TACGT-5b)5-TGCAT-3c)3-ACGTT-5d)5-ATGCA-3Correct answer is option 'D'. Can you explain this answer?.
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