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In E.coli, which component of translation machinery carries out the function for the given statements.
(i) Recognition of termination codons UAG and UAA
(ii) Recruitment of f-met - tRNA on small ribosomal subunit
(i) Recognition of termination codons UAG and UAA
(ii) Recruitment of f-met - tRNA on small ribosomal subunit
- a)RF2, IF2-GTP
- b)RF1, IF1
- c)RF1, IF2-GTP
- d)RF3, IF2-GTP
Correct answer is option 'C'. Can you explain this answer?
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Verified Answer
In E.coli, which component of translation machinery carries out the fu...
Prokaryotes have two class I release factors :-
(a) RF1– recognises stop codons UAG and UAA
(b) RF2– recognises stop codons UGA and UAA
Out of the three translation initiation factors, initiation factor 2 (IF2) is a GTP ase, which in a GTP bound state helps in the recriutment of f-met- tRNAif-met to the small ribosomal subnuit.
(a) RF1– recognises stop codons UAG and UAA
(b) RF2– recognises stop codons UGA and UAA
Out of the three translation initiation factors, initiation factor 2 (IF2) is a GTP ase, which in a GTP bound state helps in the recriutment of f-met- tRNAif-met to the small ribosomal subnuit.
Most Upvoted Answer
In E.coli, which component of translation machinery carries out the fu...
RF1 and IF2-GTP
Recognition of termination codons UAG and UAA:
- RF1 (Release Factor 1) is responsible for recognizing and binding to the termination codons UAG and UAA during translation termination in E. coli.
- RF1 triggers the release of the newly synthesized protein from the ribosome when it encounters a stop codon.
Recruitment of f-met - tRNA on small ribosomal subunit:
- IF2-GTP (Initiation Factor 2 bound to GTP) plays a crucial role in the initiation of translation by helping in the recruitment of the initiator tRNA (f-met-tRNA) to the small ribosomal subunit.
- IF2-GTP also aids in the binding of the small ribosomal subunit to the mRNA and the formation of the initiation complex.
Therefore, in E. coli, RF1 is responsible for the recognition of termination codons UAG and UAA, while IF2-GTP is involved in the recruitment of the f-met-tRNA on the small ribosomal subunit during translation.
Recognition of termination codons UAG and UAA:
- RF1 (Release Factor 1) is responsible for recognizing and binding to the termination codons UAG and UAA during translation termination in E. coli.
- RF1 triggers the release of the newly synthesized protein from the ribosome when it encounters a stop codon.
Recruitment of f-met - tRNA on small ribosomal subunit:
- IF2-GTP (Initiation Factor 2 bound to GTP) plays a crucial role in the initiation of translation by helping in the recruitment of the initiator tRNA (f-met-tRNA) to the small ribosomal subunit.
- IF2-GTP also aids in the binding of the small ribosomal subunit to the mRNA and the formation of the initiation complex.
Therefore, in E. coli, RF1 is responsible for the recognition of termination codons UAG and UAA, while IF2-GTP is involved in the recruitment of the f-met-tRNA on the small ribosomal subunit during translation.
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