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Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed that
  • a)
    both the cell lines had the same amount of radioactivity  
  • b)
    the fast growing cells had more radioactivity
  • c)
    the slow growing cells had more radioactivity
  • d)
    neither of the cells had any radioactivity
Correct answer is option 'C'. Can you explain this answer?
Most Upvoted Answer
Two mammalian cell lines with doubling times of 12 h and 36 h were cul...
Explanation:

  • The cells were cultured with radioactive thymidine for 8 hours, which means that the radioactive thymidine was incorporated into the newly synthesized DNA during this time.

  • After 8 hours, the cells were further cultured without the radioactive thymidine for 72 hours, during which time they underwent cell division and DNA replication.

  • Since the two cell lines have different doubling times, the fast-growing cells would have undergone more rounds of DNA replication during the 72-hour period than the slow-growing cells.

  • Therefore, the fast-growing cells would have incorporated more radioactive thymidine into their DNA during the 8-hour period, leading to a higher amount of radioactivity in these cells compared to the slow-growing cells.

  • Hence, option (c) is the correct answer.

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Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed thata)both the cell lines had the same amount of radioactivity b)the fast growing cells had more radioactivityc)the slow growing cells had more radioactivityd)neither of the cells had any radioactivityCorrect answer is option 'C'. Can you explain this answer?
Question Description
Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed thata)both the cell lines had the same amount of radioactivity b)the fast growing cells had more radioactivityc)the slow growing cells had more radioactivityd)neither of the cells had any radioactivityCorrect answer is option 'C'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed thata)both the cell lines had the same amount of radioactivity b)the fast growing cells had more radioactivityc)the slow growing cells had more radioactivityd)neither of the cells had any radioactivityCorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Two mammalian cell lines with doubling times of 12 h and 36 h were cultured with radioactive thymidine for 8 h. The cells were further cultured without the radioactive thymidine for 72 h. Incorporated radioactivity was measured in equal number of cells in each culture, which revealed thata)both the cell lines had the same amount of radioactivity b)the fast growing cells had more radioactivityc)the slow growing cells had more radioactivityd)neither of the cells had any radioactivityCorrect answer is option 'C'. Can you explain this answer?.
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