Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffiths experiment. They observed thata)Proteases and RNases affected transformation.b)DNase inhibited transformation.c)Proteases and Lipases affected transformation.d)RNases inhibited transformation.Correct answer is option 'B'. Can you explain this answer?

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a)
Proteases and RNases affected transformation.
b)
DNase inhibited transformation.
c)
Proteases and Lipases affected transformation.
d)
RNases inhibited transformation.

Correct answer is option 'B'. Can you explain this answer?

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Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify ...

Oswald Avery, Colin MacLeod and Maclyn McCarty. They worked to determine the biochemical nature of ‘transforming principle’ in Griffith’s experiment. They discovered that

DNA alone is transformed.
Proteases and RNases did not affect transformation.
Digestion with DNase inhibited transformation, suggesting that the DNA caused the transformation.

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Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify ...

Explanation:
In Griffith's experiment, Oswald Avery, Colin MacLeod, and Maclyn McCarty used enzymes to purify biochemicals from the heat-killed S cells in order to determine which specific biochemicals were responsible for transforming live R cells into S cells. The experiment aimed to identify the transforming principle responsible for the genetic material transfer observed in the transformation process.

Effect of Enzymes on Transformation:
The researchers used various enzymes to treat the biochemicals obtained from the heat-killed S cells and tested their ability to transform live R cells into S cells. The results of their experiment led to the following observations:

a) Proteases and RNases affected transformation:
- Proteases are enzymes that break down proteins, while RNases are enzymes that degrade RNA.
- When the researchers treated the biochemicals with proteases and RNases, they observed that transformation still occurred.
- This suggests that neither proteins nor RNA are the key transforming principle responsible for the genetic material transfer.

b) DNase inhibited transformation:
- DNase is an enzyme that degrades DNA.
- When the researchers treated the biochemicals with DNase, they observed that transformation did not occur.
- This indicates that DNA is the transforming principle responsible for the genetic material transfer.
- The presence of DNase inactivates the DNA, preventing it from transforming the live R cells into S cells.

c) Proteases and Lipases affected transformation:
- Lipases are enzymes that break down lipids (fats).
- The researchers did not observe the effect of lipases on transformation in their experiment.
- Therefore, the statement that proteases and lipases affected transformation is not supported by the given information.

d) RNases inhibited transformation:
- The researchers observed that transformation still occurred when the biochemicals were treated with RNases.
- This indicates that RNAs are not the transforming principle responsible for the genetic material transfer.
- Therefore, the statement that RNases inhibited transformation is not supported by the given information.

Based on the given information, the correct answer is option 'b' - DNase inhibited transformation. In the Griffith's experiment, the researchers found that treating the biochemicals with DNase prevented the transformation of live R cells into S cells, indicating that DNA is the transforming principle responsible for the genetic material transfer.

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Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffiths experiment. They observed thata)Proteases and RNases affected transformation.b)DNase inhibited transformation.c)Proteases and Lipases affected transformation.d)RNases inhibited transformation.Correct answer is option 'B'. Can you explain this answer?

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Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffiths experiment. They observed thata)Proteases and RNases affected transformation.b)DNase inhibited transformation.c)Proteases and Lipases affected transformation.d)RNases inhibited transformation.Correct answer is option 'B'. Can you explain this answer? for Class 12 2024 is part of Class 12 preparation. The Question and answers have been prepared according to the Class 12 exam syllabus. Information about Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffiths experiment. They observed thata)Proteases and RNases affected transformation.b)DNase inhibited transformation.c)Proteases and Lipases affected transformation.d)RNases inhibited transformation.Correct answer is option 'B'. Can you explain this answer? covers all topics & solutions for Class 12 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Oswald Avery, Colin MacLeod and Maclyn McCarty used enzymes to purify biochemicals such as proteins, DNA and RNA from the heat-killed S cells to see which ones could transform live R cells into S cells in Griffiths experiment. They observed thata)Proteases and RNases affected transformation.b)DNase inhibited transformation.c)Proteases and Lipases affected transformation.d)RNases inhibited transformation.Correct answer is option 'B'. Can you explain this answer?.

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