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Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?
- a)Hanes plot equation
- b)Eadie-Hofstee plot equation
- c)Miachelis Menten equation
- d)Eisenthal and Cornish and Bowden plot equation
Correct answer is option 'A'. Can you explain this answer?
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Most Upvoted Answer
Which of the following is based on rearrangement of Lineweaver Burk pl...
Hanes plot equation is based on rearrangement of the Lineweaver Burk plot equation by multiplication of [S0] factor and is given by
where,
V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.
The graphical representation of [S0]/V0 versus [S0] is called the Hanes plot. One of the drawbacks of this approach is that neither the ordinate nor the abscissa represents independent variable, both are dependent on substrate concentration.
where,
V0 = initial velocity, Vmax = maximum rate, Km = Miachelis Menten constant and [S] = substrate concentration.
The graphical representation of [S0]/V0 versus [S0] is called the Hanes plot. One of the drawbacks of this approach is that neither the ordinate nor the abscissa represents independent variable, both are dependent on substrate concentration.
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Which of the following is based on rearrangement of Lineweaver Burk pl...
Introduction:
The Lineweaver-Burk plot is a graphical method used to determine the kinetic parameters of an enzyme-catalyzed reaction. It is based on the Michaelis-Menten equation, which describes the relationship between the rate of an enzymatic reaction and the concentration of the substrate. The Lineweaver-Burk plot is a double reciprocal plot of the Michaelis-Menten equation.
Explanation:
The Lineweaver-Burk plot equation is given by:
1/V = (Km/Vmax) * (1/[So]) + 1/Vmax
Where:
- V is the initial velocity of the reaction
- Km is the Michaelis constant, which represents the substrate concentration at which the reaction rate is half of the maximum rate
- Vmax is the maximum velocity of the reaction
- [So] is the initial substrate concentration
Rearrangement:
To rearrange the Lineweaver-Burk plot equation, we can multiply both sides of the equation by [So]:
[So]/V = (Km/Vmax) * (1/[So]) * [So] + [So]/Vmax
Simplifying the equation gives:
[So]/V = Km/Vmax + [So]/Vmax
Conclusion:
The rearranged equation, [So]/V = Km/Vmax + [So]/Vmax, is known as the Hanes plot equation. It is obtained by multiplying the Lineweaver-Burk plot equation by the factor [So]. The Hanes plot equation is another way to represent the relationship between the substrate concentration and the initial velocity of an enzyme-catalyzed reaction. Therefore, the correct answer is option A: Hanes plot equation.
The Lineweaver-Burk plot is a graphical method used to determine the kinetic parameters of an enzyme-catalyzed reaction. It is based on the Michaelis-Menten equation, which describes the relationship between the rate of an enzymatic reaction and the concentration of the substrate. The Lineweaver-Burk plot is a double reciprocal plot of the Michaelis-Menten equation.
Explanation:
The Lineweaver-Burk plot equation is given by:
1/V = (Km/Vmax) * (1/[So]) + 1/Vmax
Where:
- V is the initial velocity of the reaction
- Km is the Michaelis constant, which represents the substrate concentration at which the reaction rate is half of the maximum rate
- Vmax is the maximum velocity of the reaction
- [So] is the initial substrate concentration
Rearrangement:
To rearrange the Lineweaver-Burk plot equation, we can multiply both sides of the equation by [So]:
[So]/V = (Km/Vmax) * (1/[So]) * [So] + [So]/Vmax
Simplifying the equation gives:
[So]/V = Km/Vmax + [So]/Vmax
Conclusion:
The rearranged equation, [So]/V = Km/Vmax + [So]/Vmax, is known as the Hanes plot equation. It is obtained by multiplying the Lineweaver-Burk plot equation by the factor [So]. The Hanes plot equation is another way to represent the relationship between the substrate concentration and the initial velocity of an enzyme-catalyzed reaction. Therefore, the correct answer is option A: Hanes plot equation.
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Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer?
Question Description
Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? for Chemistry 2024 is part of Chemistry preparation. The Question and answers have been prepared according to the Chemistry exam syllabus. Information about Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for Chemistry 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer?.
Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? for Chemistry 2024 is part of Chemistry preparation. The Question and answers have been prepared according to the Chemistry exam syllabus. Information about Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? covers all topics & solutions for Chemistry 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer?.
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Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer?, a detailed solution for Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? has been provided alongside types of Which of the following is based on rearrangement of Lineweaver Burk plot equation by multiplication of [So] factor?a)Hanes plot equationb)Eadie-Hofstee plot equationc)Miachelis Menten equationd)Eisenthal and Cornish and Bowden plot equationCorrect answer is option 'A'. Can you explain this answer? theory, EduRev gives you an
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