Which of the following is not done using column chromatography?a)The i...
Separation of inorganic cations or complexes is done using paper chromatography. Column chromatography is a method used to purify individual chemical compounds from mixtures of compounds.
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Which of the following is not done using column chromatography?a)The i...
Column Chromatography
Column chromatography is a widely used technique in chemistry for the separation and purification of mixtures. It involves the passage of a liquid mixture through a column containing a stationary phase, which separates the components based on their interactions with the stationary phase.
Not done using column chromatography
Separation of inorganic cations or complexes
Column chromatography is not typically used for the separation of inorganic cations or complexes. This is because inorganic ions or complexes do not interact strongly with the stationary phase in the column, leading to poor separation efficiency. Techniques such as ion exchange chromatography or high-performance liquid chromatography (HPLC) are more suitable for the separation of inorganic species.
Other applications of column chromatography
1. The identification of unknown compounds: Column chromatography is often used in the identification of unknown compounds by comparing their retention times with known standards.
2. The determination of homogeneity of chemical substances: Column chromatography can be used to determine the purity and homogeneity of chemical substances by separating out impurities or different components present in the sample.
3. Separation of geometric isomers: Column chromatography can be used to separate geometric isomers based on their differences in interactions with the stationary phase.
In conclusion, while column chromatography is a versatile technique with various applications in separation and purification, it is not typically used for the separation of inorganic cations or complexes due to their weak interactions with the stationary phase.