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Local conditions can affect the specificity of an enzyme for its substrate, and thus the enzymes catalytic ability: which of the following alterations would most likely not affect an enzyme in this manner?
  • a)
    Increased substrate concentration
  • b)
    Increased temperature
  • c)
    Increased concentration of OH
  • d)
    Increased concentration of H+
Correct answer is option 'A'. Can you explain this answer?
Most Upvoted Answer
Local conditions can affect the specificity of an enzyme for its subst...
Increased substrate concentration is more likely to enhance the catalytic ability of an enzyme rather than affect its specificity. When the substrate concentration is increased, the enzyme has a greater chance of encountering and binding to the substrate molecules, leading to an increase in the rate of the enzymatic reaction. However, it does not directly alter the enzyme's specificity for its substrate.
On the other hand, options B, C, and D can potentially affect the enzyme's specificity and catalytic ability:
  • Increased temperature (option B) can denature the enzyme and disrupt its active site, leading to a loss of specificity and a decrease in catalytic ability.
  • Increased concentration of OH- (option C) or H+ (option D) can alter the pH of the environment, which can affect the ionization state of amino acid residues in the enzyme's active site. This change in ionization state can impact the electrostatic interactions and binding affinity between the enzyme and substrate, thereby affecting specificity and catalytic ability.
Therefore, the alteration that would least likely affect the enzyme's specificity is option A, increased substrate concentration.
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Local conditions can affect the specificity of an enzyme for its substrate, and thus the enzymes catalytic ability: which of the following alterations would most likely not affect an enzyme in this manner?a)Increased substrate concentrationb)Increased temperaturec)Increased concentration of OH−d)Increased concentration of H+Correct answer is option 'A'. Can you explain this answer?
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