Which of the following method is used for the production of vaccines a...
The chick-embryo technique has been used in the production of vaccines against smallpox, yellow fever, influenza, and other diseases and in immunologic tests and other studies whenever large amounts of virus are required.
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Which of the following method is used for the production of vaccines a...
Chick-Embryo Method for Yellow Fever Vaccine Production:
Yellow fever vaccine is primarily produced using the chick-embryo method. This method involves the inoculation of the yellow fever virus into the fertilized eggs of specific pathogen-free (SPF) chicken embryos. The virus replicates within the chick embryos, leading to the production of viral particles that are then harvested for vaccine production.
Steps in the Chick-Embryo Method:
- Fertilized SPF chicken eggs are obtained and incubated to allow embryonic development.
- The eggs are carefully opened at a specific stage of development, and the virus is inoculated into the yolk sac or the allantoic cavity of the embryo.
- The virus replicates within the chick embryo, leading to the production of a large number of viral particles.
- The embryos are then harvested, and the viral particles are purified and inactivated to create the yellow fever vaccine.
Advantages of the Chick-Embryo Method:
- The chick-embryo method allows for large-scale production of the yellow fever vaccine.
- The method is well-established and has been used for decades in the production of various viral vaccines.
- The resulting vaccine is highly immunogenic and effective in preventing yellow fever infection.
Conclusion:
In conclusion, the chick-embryo method is the primary method used for the production of vaccines against yellow fever. This method has been instrumental in providing effective protection against yellow fever and has contributed to the control of this mosquito-borne disease.