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Using which of the following techniques can a gene be added to the chloroplast genome?
- a)Agrobacterium mediated transformation
- b)particle delivery system/Biolistics
- c)permeabilization
- d)Electroporation
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
Using which of the following techniques can a gene be added to the chl...
Concept:
- Biolistics, or particle bombardment, is a common method for nuclear plant transformation, and for transformations that require DNA to be delivered to chloroplasts and mitochondria.
Explanation:
Fig 1: Biolistic transformation
- Biolistics is based on the direct delivery of DNA into plant cells using gold or tungsten particles.
- The gold or tungsten particles are coated with DNA which are shot at plant tissue at high velocity and can become lodged inside plant cells.
- Once inside the cell, the DNA elutes off the particles and becomes transiently expressed or stably integrates into the host genome.
- The physical delivery of DNA to cells circumvents host-range limitations, as is sometimes encountered with Agrobacterium.
- Biolistics has been employed to successfully deliver genome-editing reagents in multiple crop plants, including corn, cotton, soybean, and wheat.
hence the correct answer is option 1
Most Upvoted Answer
Using which of the following techniques can a gene be added to the chl...
Particle Delivery System/Biolistics for Adding Genes to Chloroplast Genome
- This technique involves the use of small particles coated with the gene of interest to deliver it into the chloroplast genome.
- The gene of interest is first coated onto tiny particles such as gold or tungsten.
- These particles are then shot into plant cells using a gene gun or particle delivery system.
- The high velocity of the particles allows them to penetrate the cell wall and deliver the gene into the chloroplast genome.
- Once inside the chloroplast, the gene integrates into the chloroplast genome and is expressed, leading to the desired trait or protein production.
This technique is particularly useful for adding genes to the chloroplast genome because chloroplasts have a unique genetic system separate from the nuclear genome. Biolistics allows for the direct delivery of genes into the chloroplast, bypassing the need for complex transformation methods.
- This technique involves the use of small particles coated with the gene of interest to deliver it into the chloroplast genome.
- The gene of interest is first coated onto tiny particles such as gold or tungsten.
- These particles are then shot into plant cells using a gene gun or particle delivery system.
- The high velocity of the particles allows them to penetrate the cell wall and deliver the gene into the chloroplast genome.
- Once inside the chloroplast, the gene integrates into the chloroplast genome and is expressed, leading to the desired trait or protein production.
This technique is particularly useful for adding genes to the chloroplast genome because chloroplasts have a unique genetic system separate from the nuclear genome. Biolistics allows for the direct delivery of genes into the chloroplast, bypassing the need for complex transformation methods.
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Using which of the following techniques can a gene be added to the chloroplast genome?a)Agrobacterium mediated transformationb)particle delivery system/Biolisticsc)permeabilizationd)ElectroporationCorrect answer is option 'B'. Can you explain this answer?
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Using which of the following techniques can a gene be added to the chloroplast genome?a)Agrobacterium mediated transformationb)particle delivery system/Biolisticsc)permeabilizationd)ElectroporationCorrect answer is option 'B'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about Using which of the following techniques can a gene be added to the chloroplast genome?a)Agrobacterium mediated transformationb)particle delivery system/Biolisticsc)permeabilizationd)ElectroporationCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Using which of the following techniques can a gene be added to the chloroplast genome?a)Agrobacterium mediated transformationb)particle delivery system/Biolisticsc)permeabilizationd)ElectroporationCorrect answer is option 'B'. Can you explain this answer?.
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