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Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody by
- a)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.
- b)replacing the Fv region of a mAb with one derived from a human IgG.
- c)replacing CL and CH regions of the mAb with that obtained from a human IgG.
- d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
Monoclonal antibodies (mAb) can be potentially used as therapeutic age...
Concept:
- Monoclonal antibodies (mAbs) represent effective therapeutic agents as they demonstrate significant specificity for their targets and confer effector functions such as receptor-ligand blockade, target cell cytotoxicity and receptor antagonism.
- However, the use of mAbs in clinical settings has been complicated by a number of technical challenges including the demonstration of immunogenic responses.
- Immunogenic responses to antibody therapeutics can impact both safety and pharmacokinetic properties which can impact utility and efficacy of the drugs.
- A heightened awareness of these issues has developed as the field matures and the clinical consequences of immune responses to therapeutics have been reported and detailed.
Explanation:
Fig 1: CDRs of Antibody
- Initially it was less apparent that injecting patients with purified mouse-derived antibodies, i.e., murine mAbs, could also cause immune reactions.
- An appreciation of the consequences of an immune response to murine antibodies has lead to the development of engineered antibody constructs that carry a lower risk of immune reactions.
- Engineering of antibodies by sequentially replacing mouse sequence-derived amino acids for human sequences has in fact significantly reduced immunogenicity of this class of therapeutics.
- Chimeric antibodies were the first engineered improvement where the murine constant regions were replaced by human constant regions.
- The next development was the humanization process.
- Humanization results in an antibody where only the complementarity determining regions (CDRs) of the variable (V) regions are of mouse-sequence origin.
- The current state of the art is fully human amino acid sequence derived antibody region therapeutics where antigen specificity has been selected either in vivo by the use of genetically modified mice or by antibody engineering processes combined with screening.
- Fully human and humanized antibodies carry a lower risk for inducing immune responses in humans than mouse or chimeric antibodies.
Option 1:
expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.
- Consider the explanation above thus this option is not true, as to generate monoclonal antibodies the animal has to be injected with antigen which is unethical in human subjects.
Option 2:
replacing the Fv region of a mAb with one derived from a human IgG.
- Consider the explanation above thus this option is not true
Option 3:
replacing C
L
and C
H
regions of the mAb with that obtained from a human IgG.
- Consider the explanation above thus this option is not true
Option 4:
taking a human IgG and replacing the CDRS by those derived from the mouse mAb.
- Consider the explanation above thus this option is true, as humanization of an antibody with only complementarity determining regions (CDRs) of the variable (V) regions are of mouse-sequence origin makes immune respose to it very negligible.
hence the correct answer is option 4
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Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer?
Question Description
Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer?.
Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer?.
Solutions for Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? in English & in Hindi are available as part of our courses for Software Development.
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Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer?, a detailed solution for Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? has been provided alongside types of Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice Monoclonal antibodies (mAb) can be potentially used as therapeutic agents. The major advantage is that they can specifically target aberrant cells. However, there is a practical difficulty. Monoclonals are raised in mouse and therefore it is expected that an immune reaction will develop if these are injected into humans. It is therefore necessary to humanize monoclonal antibody bya)expressing the genes for the mAb in cultured human cells and isolating the mAb from these cells.b)replacing the Fv region of a mAb with one derived from a human IgG.c)replacing CL and CH regions of the mAb with that obtained from a human IgG.d)taking a human IgG and replacing the CDRS by those derived from the mouse mAb.Correct answer is option 'D'. Can you explain this answer? tests, examples and also practice Software Development tests.
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