In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)Which one is the correct sequence of the presence of this protein upon oligopeptide?a)Arg-Ala-Ser-Lys-Met-Phe-Ser-Alab)Arg-Ala-Ser-Lys-Phe-Met-Ser-Alac)Ala-Arg-Ser-Phe-Lys-Met-Ser-Alad)Ala-Arg-Phe-Ser-Lys-Met-Ser-AlaCorrect answer is option 'B'. Can you explain this answer?

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In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):
(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)
(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala
(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)
(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)
(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)
Which one is the correct sequence of the presence of this protein upon oligopeptide?

a)
Arg-Ala-Ser-Lys-Met-Phe-Ser-Ala
b)
Arg-Ala-Ser-Lys-Phe-Met-Ser-Ala
c)
Ala-Arg-Ser-Phe-Lys-Met-Ser-Ala
d)
Ala-Arg-Phe-Ser-Lys-Met-Ser-Ala

Correct answer is option 'B'. Can you explain this answer?

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In order to determine the primary structure of an octapeptide, the ami...

Explanation:

Given Information:
- Amino acid composition after acid hydrolysis: (2Ala,Arg,Lys,Met,Phe, 2Ser)
- Results after treatment with various enzymes and subsequent acid hydrolysis

Analysis:
- Carboxypeptidase (B): (Ala,Arg,Lys,Met,Phe,2Ser) after acid hydrolysis
- Chymotrypsin (C): (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser) after acid hydrolysis
- Trypsin (D): (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser) after acid hydrolysis
- CNBr (E): (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser) after acid hydrolysis

Sequence Determination:
- From the given information and enzyme treatments, we can deduce the sequence as follows:
- B gives Ala at the N-terminal
- C gives Arg at the C-terminal
- D gives Met at the C-terminal
- E gives Phe at the C-terminal

Correct Sequence:
- Based on the above deductions and information, the correct sequence of the octapeptide is:
- Arg-Ala-Ser-Lys-Met-Phe-Ser-Ala
Therefore, option B (Arg-Ala-Ser-Lys-Phe-Met-Ser-Ala) is the correct sequence based on the given data and enzyme treatments.

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In order to determine the primary structure of an octapeptide, the ami...

Acid hydrolysis is a method used to hydrolyze proteins into amino acids. In the case of A only acid, hydrolysis is carried out and thus it represents the constituent amino acids of the protein. In all other cases, acid hydrolysis is followed by a particular treatment. The carboxypeptidase removes the C-terminal amino acid and in this case, it is an alanine residue, as in B an alanine is separated from all other amino acids. Chymotrypsin cleaves the aromatic amino acids and probably, in this case, it is phenylalanine in C, we can see that there are four amino acids namely: Ala, Lys, Met, and Ser are separated from the phenylalanine set indicating that they come after the phenylalanine. In D, which is treated with trypsin cleavage is occurring between the carboxyl group of arginine or lysine and the amino group of the adjacent amino acid. It gives three fragments indicating that two cleavages had occurred. The first cleavage is between Arg resulting in a small fragment of alanine and arginine and the second is involved between Lys which yields two fragments. In E, the treatment with CNBr is carried out. Cyanogen Bromide or CNBr cleaves methionine residues and in this case, only an Alanine and Serine is separated. Altogether, we can say that from the C terminal the order of amino acids will be Ala-Ser-Met-Lys-Phe-Ser-Arg-Ala, which is option C. The cleavage patterns are depicted as follows:

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In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)Which one is the correct sequence of the presence of this protein upon oligopeptide?a)Arg-Ala-Ser-Lys-Met-Phe-Ser-Alab)Arg-Ala-Ser-Lys-Phe-Met-Ser-Alac)Ala-Arg-Ser-Phe-Lys-Met-Ser-Alad)Ala-Arg-Phe-Ser-Lys-Met-Ser-AlaCorrect answer is option 'B'. Can you explain this answer?

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In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)Which one is the correct sequence of the presence of this protein upon oligopeptide?a)Arg-Ala-Ser-Lys-Met-Phe-Ser-Alab)Arg-Ala-Ser-Lys-Phe-Met-Ser-Alac)Ala-Arg-Ser-Phe-Lys-Met-Ser-Alad)Ala-Arg-Phe-Ser-Lys-Met-Ser-AlaCorrect answer is option 'B'. Can you explain this answer? for Software Development 2024 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)Which one is the correct sequence of the presence of this protein upon oligopeptide?a)Arg-Ala-Ser-Lys-Met-Phe-Ser-Alab)Arg-Ala-Ser-Lys-Phe-Met-Ser-Alac)Ala-Arg-Ser-Phe-Lys-Met-Ser-Alad)Ala-Arg-Phe-Ser-Lys-Met-Ser-AlaCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for Software Development 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In order to determine the primary structure of an octapeptide, the amino acid composition was determined by acid hydrolysis (A). The intact oligopeptide was treated with carboxypeptidase (B), chymotrypsin (C), trypsin (D), and CNBr (E). The peptides were separated in each case and acid hydrolysis was carried out for B -E. The following results were obtained (the brackets represent mixtures of amino acids in each fragment):(A) (2Ala,Arg,Lys,Met,Phe, 2Ser)(B) (Ala,Arg,Lys,Met,Phe,2Ser)and Ala(C) (Ala,Arg,Phe,Ser),(Ala,Lys,Met,Ser)(D) (Ala,Arg),(Lys,Phe,Ser),(Ala,Met,Ser)(E) (Ala,Arg,Lys,Met,Phe,Ser),(Ala,Ser)Which one is the correct sequence of the presence of this protein upon oligopeptide?a)Arg-Ala-Ser-Lys-Met-Phe-Ser-Alab)Arg-Ala-Ser-Lys-Phe-Met-Ser-Alac)Ala-Arg-Ser-Phe-Lys-Met-Ser-Alad)Ala-Arg-Phe-Ser-Lys-Met-Ser-AlaCorrect answer is option 'B'. Can you explain this answer?.

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