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If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?
  • a)
    The DNA will gradually increase in length till it is fully replicated
  • b)
    Small fragments of DNA will be obtained increasing in number with time
  • c)
    A mixture of small and long Fragments of definite length from the start whose concentration simply increases with time
  • d)
    At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite length
Correct answer is option 'D'. Can you explain this answer?
Verified Answer
If we mutate the DNA ligase and observe the length of the replicated s...
If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite length.
The small fragments are the Okazaki fragments of the lagging strand while the longer is the leading strand DNA. While the leading strand grows in length the Okazaki fragments in absence of ligation to each other result in small fragments of definite length.
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Most Upvoted Answer
If we mutate the DNA ligase and observe the length of the replicated s...
Explanation:

Background:
DNA ligase is an enzyme that plays a crucial role in the process of DNA replication by catalyzing the formation of phosphodiester bonds between adjacent nucleotides, thereby joining the Okazaki fragments on the lagging strand.

Observation:
When we mutate the DNA ligase and observe the length of replicated DNA strands in different time slots after replication initiation, we will observe the following:

Fragment Length:
- Initially, small fragments of DNA will be obtained due to the inability of the mutated DNA ligase to efficiently join the Okazaki fragments on the lagging strand.
- As replication progresses, the number of small fragments will increase with time, indicating incomplete replication.

Distinct Bands:
- Over time, we may observe two separate bands on the gel electrophoresis results.
- One band will represent long fragments with increasing length as the leading strand is replicated continuously.
- The second band will consist of short fragments of a definite length, reflecting the inability of the mutated DNA ligase to properly ligate the Okazaki fragments on the lagging strand.

Conclusion:
Therefore, the observation of small fragments initially, followed by two separate bands showing long fragments of increasing length and short fragments of a definite length, indicates the impact of mutating DNA ligase on DNA replication dynamics.
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If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?a)The DNA will gradually increase in length till it is fully replicatedb)Small fragments of DNA will be obtained increasing in number with timec)A mixture of small and long Fragments of definite length from the start whose concentration simply increases with timed)At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite lengthCorrect answer is option 'D'. Can you explain this answer?
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If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?a)The DNA will gradually increase in length till it is fully replicatedb)Small fragments of DNA will be obtained increasing in number with timec)A mixture of small and long Fragments of definite length from the start whose concentration simply increases with timed)At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite lengthCorrect answer is option 'D'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?a)The DNA will gradually increase in length till it is fully replicatedb)Small fragments of DNA will be obtained increasing in number with timec)A mixture of small and long Fragments of definite length from the start whose concentration simply increases with timed)At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite lengthCorrect answer is option 'D'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for If we mutate the DNA ligase and observe the length of the replicated strands in different time slots after replication initiation, what will we observe?a)The DNA will gradually increase in length till it is fully replicatedb)Small fragments of DNA will be obtained increasing in number with timec)A mixture of small and long Fragments of definite length from the start whose concentration simply increases with timed)At first small fragment, then two separate bands showing long fragments with increasing length and short fragments of definite lengthCorrect answer is option 'D'. Can you explain this answer?.
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