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In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?
- a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase I
- b)DNA a, DNA b, HU, SSB, polymerase III
- c)DNA a, DNA b, DNA c, HU, SSB polymerase I
- d)DNA a, DNA b, DNA c, HU, SSB, polymerase III
Correct answer is option 'C'. Can you explain this answer?
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In an experiment, you take DNA in vitro and attempt to replicate it. W...
In an experiment, you take DNA in vitro and attempt to replicate it. DNA a, DNA b, DNA c, HU, SSB polymerase I combination you will add to your DNA to get the maximum replication product.
DNA pol I have primase activity which is mandatory for starting DNA replication. As pol III lacks primase activity it will not be able to initiate replication. Also in vitro (especially in open DNA strands) topoisomerase doesn’t have much role to play but DNA c is a must for replication as it loads the helicase.
DNA a is a protein that activates the initiation of DNA replication in bacteria. It is a replication initiation factor that promotes the unwinding of DNA at oriC. The onset of the initiation phase of DNA replication is determined by the concentration of DNA a.
DNA b helicase is an enzyme in bacteria that opens the replication fork during DNA replication. Although the mechanism by which DNA b both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerization of the N-terminal domain has been observed and may occur during the enzymatic cycle.
The HU protein is a small, basic, heat-stable DNA-binding protein that is well-conserved in prokaryotes and is associated with the bacterial nucleoid. HU was shown to participate in vitro in the initiation of DNA replication as an accessory factor to assist the action of DNA a protein in the unwinding of oriC DNA.
DNA pol I have primase activity which is mandatory for starting DNA replication. As pol III lacks primase activity it will not be able to initiate replication. Also in vitro (especially in open DNA strands) topoisomerase doesn’t have much role to play but DNA c is a must for replication as it loads the helicase.
DNA a is a protein that activates the initiation of DNA replication in bacteria. It is a replication initiation factor that promotes the unwinding of DNA at oriC. The onset of the initiation phase of DNA replication is determined by the concentration of DNA a.
DNA b helicase is an enzyme in bacteria that opens the replication fork during DNA replication. Although the mechanism by which DNA b both couples ATP hydrolysis to translocation along DNA and denatures the duplex is unknown, a change in the quaternary structure of the protein involving dimerization of the N-terminal domain has been observed and may occur during the enzymatic cycle.
The HU protein is a small, basic, heat-stable DNA-binding protein that is well-conserved in prokaryotes and is associated with the bacterial nucleoid. HU was shown to participate in vitro in the initiation of DNA replication as an accessory factor to assist the action of DNA a protein in the unwinding of oriC DNA.
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In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer?
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In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer?.
In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? for Software Development 2025 is part of Software Development preparation. The Question and answers have been prepared according to the Software Development exam syllabus. Information about In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? covers all topics & solutions for Software Development 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer?.
Solutions for In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? in English & in Hindi are available as part of our courses for Software Development.
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In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer?, a detailed solution for In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? has been provided alongside types of In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? theory, EduRev gives you an
ample number of questions to practice In an experiment, you take DNA in vitro and attempt to replicate it. Which combination will you add to your DNA to get the maximum replication product?a)DNA a, DNA b, HU, SSB, topoisomerase I, polymerase Ib)DNA a, DNA b, HU, SSB, polymerase IIIc)DNA a, DNA b, DNA c, HU, SSB polymerase Id)DNA a, DNA b, DNA c, HU, SSB, polymerase IIICorrect answer is option 'C'. Can you explain this answer? tests, examples and also practice Software Development tests.
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