Which of the following is a comparatively significant factor affecting...
The correct answer is Option C.
The specific rotation of a molecule is the rotation in degrees observed upon passing polarized light through a path length of 1 decimetre (dm) at a concentration of 1 g/mL. Specific rotation is almost always reported along with the temperature, wavelength of light used, the solvent, and the concentration, since it is sensitive to these factors as well.
Magnitude of specific rotation depends on temperature. However, it does not depend on concentration, purity or length of sample tude.
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Which of the following is a comparatively significant factor affecting...
Yup.... Answer c is correct bcoz temperature let the magnitude in its direction..... This its the significant factor that affect optical rotation
Which of the following is a comparatively significant factor affecting...
The specific optical rotation is a property of chiral substances, which means that they have the ability to rotate the plane of polarized light. The magnitude of specific optical rotation is influenced by several factors, including the concentration of the substance, the purity of the sample, the experimental temperature, and the length of the sample tube. However, among these factors, the experimental temperature is considered to be a comparatively significant factor affecting the magnitude of specific optical rotation.
- Experimental Temperature: The specific optical rotation of a substance is temperature-dependent. As the temperature increases, the specific optical rotation may change due to the alteration of molecular interactions and the conformational changes of the chiral molecules. Therefore, it is important to control the temperature during optical rotation measurements to obtain accurate and reliable results.
- Concentration of the Substance: The concentration of the substance of interest can also affect the magnitude of specific optical rotation. In general, the specific optical rotation is directly proportional to the concentration of the chiral substance. Higher concentrations of the substance will result in a greater rotation of the polarized light. However, the concentration is not as significant as temperature in determining the magnitude of specific optical rotation.
- Purity of the Sample: The purity of the sample can also influence the specific optical rotation. Impurities or contaminants in the sample can interact with the chiral molecules and affect their ability to rotate the plane of polarized light. Therefore, it is important to use pure samples to obtain accurate measurements of specific optical rotation. However, the purity of the sample is not as significant as temperature in determining the magnitude of specific optical rotation.
- Length of Sample Tube: The length of the sample tube used in the optical rotation measurement can affect the observed rotation angle. However, this factor is typically accounted for during measurements by using standardized sample tube lengths. Therefore, while the length of the sample tube can have an impact on the specific optical rotation, it is not considered as significant as temperature.
In conclusion, among the factors listed, the experimental temperature is considered to be a comparatively significant factor affecting the magnitude of specific optical rotation. Temperature changes can lead to alterations in molecular interactions and conformational changes, which in turn affect the ability of chiral substances to rotate the plane of polarized light.
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