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Which of the following amino acid(s) move towards cathode in the electrophoresis at pH = 5?
- a)Histidine
- b)Arginine
- c)Aspartic acid
- d)Glutamic acid
Correct answer is option 'A,B'. Can you explain this answer?
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Most Upvoted Answer
Which of the following amino acid(s) move towards cathode in the elect...
Amino acids and Electrophoresis:
Electrophoresis is a technique used to separate charged molecules based on their size and charge. Amino acids can be separated by electrophoresis, as they have a charged functional group at pH values below their pKa.
pH and Electrophoresis:
The movement of an amino acid in electrophoresis depends on the pH of the medium. At different pH values, the amino acids have different charges, and hence, they move in different directions during electrophoresis.
In electrophoresis at pH = 5, the amino acids have different charges based on their pKa values. The acidic amino acids like Aspartic acid and Glutamic acid are negatively charged at pH = 5, and they move towards the anode, which is the positive electrode. The basic amino acids like Arginine and Histidine are positively charged at pH = 5, and they move towards the cathode, which is the negative electrode.
Answer Explanation:
The options given in the question are:
a) Histidine
b) Arginine
c) Aspartic acid
d) Glutamic acid
At pH = 5, the acidic amino acids Aspartic acid and Glutamic acid are negatively charged, and they move towards the anode. Hence, options c and d are incorrect.
The basic amino acids Histidine and Arginine are positively charged at pH = 5, and they move towards the cathode. Hence, options a and b are correct.
Therefore, the correct answer is option 'A,B'.
Electrophoresis is a technique used to separate charged molecules based on their size and charge. Amino acids can be separated by electrophoresis, as they have a charged functional group at pH values below their pKa.
pH and Electrophoresis:
The movement of an amino acid in electrophoresis depends on the pH of the medium. At different pH values, the amino acids have different charges, and hence, they move in different directions during electrophoresis.
In electrophoresis at pH = 5, the amino acids have different charges based on their pKa values. The acidic amino acids like Aspartic acid and Glutamic acid are negatively charged at pH = 5, and they move towards the anode, which is the positive electrode. The basic amino acids like Arginine and Histidine are positively charged at pH = 5, and they move towards the cathode, which is the negative electrode.
Answer Explanation:
The options given in the question are:
a) Histidine
b) Arginine
c) Aspartic acid
d) Glutamic acid
At pH = 5, the acidic amino acids Aspartic acid and Glutamic acid are negatively charged, and they move towards the anode. Hence, options c and d are incorrect.
The basic amino acids Histidine and Arginine are positively charged at pH = 5, and they move towards the cathode. Hence, options a and b are correct.
Therefore, the correct answer is option 'A,B'.
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Community Answer
Which of the following amino acid(s) move towards cathode in the elect...
Among them, A & B are the basic amino acid, and C & D are the acidic amino acid.
at pH 5 i.e at acidic medium, basic amino acid gets protonated, and becomes positively charged, hence they moves towards cathode.
at pH 5 i.e at acidic medium, basic amino acid gets protonated, and becomes positively charged, hence they moves towards cathode.
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Which of the following amino acid(s) move towards cathode in the electrophoresis at pH = 5?a)Histidineb)Argininec)Aspartic acid d)Glutamic acidCorrect answer is option 'A,B'. Can you explain this answer?
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