6. To study Binary Fission in Amoeba and Binary Fission & Budding in Yeast- Lab Exp. Video Lecture - Class 10

to study binary fission in amoeba and
binary fission and budding in East
a binary fission in amoeba materials
required pond water in a beaker slide
dropper coverslip compound microscope
procedure take a small drop of pond
water on a slide and cover it with a
cover slip
do not add any stain observe it under a
low power of microscope observations
amoeba are usually irregular in shape
some of them may be in a state of binary
fission in some stages gary o kindnesses
may be seen others may show the division
of cell body that is cytokinesis so that
an amoeba may be divided into two parts
that is daughter cells
be binary fission and budding in East
materials required east powder 10% sugar
solution in a flask cotton wool slide
cover slip compound microscope and
dropper procedure add 5 grams of east
powder to about 250 milliliter of 10%
sugar solution in a flask
the mouth of the flask should be tightly
plugged with cotton wool keep the flask
in a warm place for about 4 days
after four days take a small drop of the
culture solution on a slide and cover it
with the coverslip
do not add any stain observe it under
the low power of microscope
observations East cells are usually
spherical or oval in shape some may be
in a state of binary fission
others may show protuberances on them
called buds the buds after gaining
maturity separates from the parent cell
this process of reproduction is known as
budding sometimes a chain of buds is
seen on the parent cell