NEET > Biology Class 12 > Restriction Enzymes (Recognition Sequence)
Restriction Enzymes (Recognition Sequence) Video Lecture - Biology Class 12 - NEET
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FAQs on Restriction Enzymes (Recognition Sequence) Video Lecture - Biology Class 12 - NEET
| 1. What are restriction enzymes? |  |
Ans. Restriction enzymes, also known as restriction endonucleases, are proteins that can recognize specific DNA sequences and cleave the DNA at or near those sequences. They are an essential tool in molecular biology and biotechnology.
| 2. How do restriction enzymes recognize their target sequences? | |
Ans. Restriction enzymes recognize specific DNA sequences, known as recognition sequences, by binding to complementary base pairs. These enzymes have a specific binding site that matches the target sequence, allowing them to identify and bind to the DNA.
| 3. What is the significance of restriction enzymes in genetic engineering? | |
Ans. Restriction enzymes play a crucial role in genetic engineering as they allow scientists to cut DNA at specific locations. This ability to cleave DNA at precise sites enables the manipulation of DNA fragments, such as inserting or removing specific genes, which is essential for creating recombinant DNA molecules.
| 4. Are restriction enzymes found naturally in organisms? | |
Ans. Yes, restriction enzymes are naturally found in bacteria and archaea. They are part of the immune system of these organisms, protecting them against viral infections. By cleaving the viral DNA at specific sites, the restriction enzymes prevent the replication of the virus within the host cell.
| 5. Can restriction enzymes recognize and cut any DNA sequence? | |
Ans. No, restriction enzymes can only recognize and cut specific DNA sequences that match their recognition sites. Each restriction enzyme has a unique recognition sequence, typically consisting of 4 to 8 base pairs. Therefore, it is crucial to choose the appropriate restriction enzyme for a specific DNA manipulation based on the recognition sequence requirements.
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