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What is the process of blotting? What is the difference between southern and Northern blotting?
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In molecular biology and genetics, various blotting techniques are employed to detect and study changing levels of proteins, DNA, or RNA, and also to study the interactions occurring between them. Southern blotting is used in case of DNA, Northern blotting in case of RNA, Western blotting in case of proteins, and Eastern blotting in case of post-translational modifications of proteins. The purpose of each technique may be different, but all share the same principle and methodology, with a few minor deviations and modifications.
Principle
All blotting techniques are based on the same principle of specific base pairing of the probe sequences to the sequences immobilized on the membrane. Any change in the sample sequences will lead to the probes not binding or binding non-specifically. This change in binding affinity will be visualized upon secondary hybridization with a labeled probe. The intensity of the resultant dye or fluorescence will indicate the specificity of the binding along with other characteristics such as copy number, gene expression, etc.
Northern and Southern Blotting Techniques
Northern blotting is a technique used to detect and study specific RNA molecules from a mixture of different RNA, all isolated from a particular tissue or cell type. It allows the investigator to determine the molecular weight of mRNA, and also to determine the relative quantity of mRNA (gene expression) across different samples.
Southern blotting is used to detect and study specific DNA sequences. It allows one to study restricted (cut) DNA fragments, changes in the sequence, and its relative quantity across different samples.
FAQs on BLOTTING - NEET
| 1. What is the NEET exam? |  |
Ans. The NEET (National Eligibility cum Entrance Test) is a national-level medical entrance exam conducted in India for admission to undergraduate medical courses (MBBS/BDS) in government and private medical colleges.
| 2. How can I apply for the NEET exam? | |
Ans. To apply for the NEET exam, candidates need to visit the official website of the National Testing Agency (NTA) and complete the online registration process. The application form includes filling personal details, uploading documents, and paying the application fee.
| 3. What is the eligibility criteria for the NEET exam? | |
Ans. The eligibility criteria for the NEET exam include: (1) candidates must be at least 17 years old on or before December 31 of the year of admission, (2) candidates must have completed their 10+2 or equivalent examination with Physics, Chemistry, Biology/Biotechnology, and English as core subjects, and (3) candidates must have scored a minimum aggregate of 50% in the qualifying examination (45% for reserved category candidates).
| 4. What is the exam pattern of the NEET exam? | |
Ans. The NEET exam is a pen and paper-based test consisting of 180 multiple-choice questions (MCQs). The exam is divided into three sections: Physics, Chemistry, and Biology (Botany and Zoology). Each section contains 45 questions, and each correct answer carries 4 marks, while 1 mark is deducted for each incorrect answer.
| 5. How can I prepare for the NEET exam effectively? | |
Ans. To prepare effectively for the NEET exam, candidates should create a study schedule, understand the exam syllabus and pattern, practice previous years' question papers and mock tests, refer to standard textbooks and study materials, and seek guidance from experienced mentors or coaching institutes. Regular revision and self-assessment are essential for achieving good results in the exam.
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Jan 16, 2025
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Document Description: BLOTTING for NEET 2025 is part of NEET preparation. The notes and questions for BLOTTING have been prepared according to the NEET exam syllabus. Information about BLOTTING covers topics like and BLOTTING Example, for NEET 2025 Exam. Find important definitions, questions, notes, meanings, examples, exercises and tests below for BLOTTING.
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