NEET Exam > NEET Questions > What is the process of blotting? What is the ...
Start Learning for Free
What is the process of blotting? What is the difference between southern and Northern blotting?
Most Upvoted Answer
What is the process of blotting? What is the difference between southe...
Blotting Process
Blotting is a laboratory technique used to transfer DNA, RNA, or protein molecules from a gel to a membrane. The process involves three basic steps:
- Separation of molecules: The molecules of interest are separated using gel electrophoresis.
- Transfer to a membrane: The separated molecules are transferred to a membrane, typically made of nitrocellulose or nylon.
- Detection: The transferred molecules are detected using probes or antibodies that bind specifically to the molecule of interest.
Southern Blotting vs Northern Blotting
Southern blotting and Northern blotting are two variations of the blotting technique that are used to detect DNA and RNA molecules, respectively.
Southern Blotting
Southern blotting is used to detect specific DNA sequences in a sample. The process involves the following steps:
- DNA is extracted from the sample of interest and digested with restriction enzymes to produce fragments of varying lengths.
- The DNA fragments are separated by size using gel electrophoresis.
- The separated DNA fragments are transferred to a nitrocellulose or nylon membrane using a process called capillary transfer.
- A labeled DNA probe is added to the membrane, which hybridizes with the complementary DNA sequence of interest.
- The location of the labeled probe on the membrane is detected using autoradiography or chemiluminescence.
Southern blotting is commonly used in molecular biology research to detect gene mutations, to quantify gene expression, and to study DNA methylation patterns.
Northern Blotting
Northern blotting is used to detect specific RNA sequences in a sample. The process is similar to Southern blotting, but with a few key differences:
- RNA is extracted from the sample of interest and separated by size using gel electrophoresis.
- The separated RNA fragments are transferred to a nitrocellulose or nylon membrane using a process called capillary transfer.
- A labeled RNA probe is added to the membrane, which hybridizes with the complementary RNA sequence of interest.
- The location of the labeled probe on the membrane is detected using autoradiography or chemiluminescence.
Northern blotting is commonly used in molecular biology research to detect gene expression levels and to study RNA splicing patterns.
Community Answer
What is the process of blotting? What is the difference between southe...
|
Explore Courses for NEET exam
|
|
Similar NEET Doubts
Top Courses for NEETView all
What is the process of blotting? What is the difference between southern and Northern blotting?
Question Description
What is the process of blotting? What is the difference between southern and Northern blotting? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about What is the process of blotting? What is the difference between southern and Northern blotting? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for What is the process of blotting? What is the difference between southern and Northern blotting?.
What is the process of blotting? What is the difference between southern and Northern blotting? for NEET 2025 is part of NEET preparation. The Question and answers have been prepared according to the NEET exam syllabus. Information about What is the process of blotting? What is the difference between southern and Northern blotting? covers all topics & solutions for NEET 2025 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for What is the process of blotting? What is the difference between southern and Northern blotting?.
Solutions for What is the process of blotting? What is the difference between southern and Northern blotting? in English & in Hindi are available as part of our courses for NEET.
Download more important topics, notes, lectures and mock test series for NEET Exam by signing up for free.
Here you can find the meaning of What is the process of blotting? What is the difference between southern and Northern blotting? defined & explained in the simplest way possible. Besides giving the explanation of
What is the process of blotting? What is the difference between southern and Northern blotting?, a detailed solution for What is the process of blotting? What is the difference between southern and Northern blotting? has been provided alongside types of What is the process of blotting? What is the difference between southern and Northern blotting? theory, EduRev gives you an
ample number of questions to practice What is the process of blotting? What is the difference between southern and Northern blotting? tests, examples and also practice NEET tests.
|
|
Explore Courses for NEET exam
|
|
Signup for Free!
Signup to see your scores go up within 7 days! Learn & Practice with 1000+ FREE Notes, Videos & Tests.
|
© EduRev
|
Education Revolution
|
|
Signup to see your scores
go up within 7 days!
Access 1000+ FREE Docs, Videos and Tests
Takes less than 10 seconds to signup