Naming of Restriction Endonucleases Video Lecture | Biology Class 12 - NEET

hello friends this video on
biotechnology principles part eight is
brought to you by exam feel calm no more
fear from exam so now let us talk about
the name move of restriction
endonuclease because you already saw eco
r1 BAM each one to such few names
so how do we name these restriction
endonucleases now every restriction
endonuclease enzyme will have their
first later derived from the genus like
every every restriction endonuclease as
i said that end restriction enzymes are
found in the bacteria so depending upon
the particular bacteria from which that
particular restriction endonuclease
enzymes being extracted they are named
so their first letter will always
represent the genus to which the
bacteria belongs the second and third
title will represent the species of that
particular bacterium so let us take the
example of eco RI so if you see this is
how we write it eco RI so what is e e is
for the genus that is is Kancha
so E is for s Collegium see who else for
coli so ecoli is the particular
bacterium since to the vasila Joey D
instead of code is ego so second and
third leg electron them species what
about the fourth letter so the fourth
letter represent the strain of the
bacteria so here you have R so R would
represent the rough strain that means
rough strain of e.coli bacteria and
finally there is a Roman number here you
have one and what does this one indicate
it indicates the order in which the
enzymes were isolated that means this
was the first enzyme can be isolated
from eco line so it is eco r1 so this is
how all the restriction endonucleases
are named
now currently we have more than 900
restriction enzymes which
being isolated from almost over 200
strains of bacteria so you see if you do
not have a very specific naming
convention then it becomes very
difficult to distinguish between the
different restriction indonesians ahead
restriction enzymes 900 is a big number
so we have so many enzymes and that do
they have all been derived from some 200
strains of bacteria so that is the
reason why we follow such a specific a
convention of nailing the enzymes so
each of these enzymes will have a
specific recognition sequence so let us
look at some of the other examples of
restriction endonuclease enzyme so when
you say eco RI so that's the darwin
that's what I told you just know so this
eco r1 is derived or extracted from is
very choco light so that is why e and
then quote from Kota you let's see oh
so it is equal R was the strain that is
the rough strain and one is the first
enzyme which was extracted from equal
egg next is bam h1 so here BAM and D is
from
basileus and ileum is from am i doing
liquidations so this is the bacteria
from which bam h1 is derived and H
represents the strain and one ends for
the first enzyme which have been
extracted
third is hint to so this is from this
bacteria that is Haemophilus so from
here that gets age and I n so that is
how it is H I N and D is the strain and
2 represents down second back second
enzyme which was extracted from hepa
filters and provenza then san1 so here
is estin sports petrol prices al is for
Albers so sad one one would mean
first enzyme which have been extracted
from streptomyces anchors so this is how
all the restriction endonucleases are
named and that is why you find their
names little weird but these do remember
that each restriction endonuclease will
have a specific declination sequence so
each of them will have one specific
sequence so they will also cut the
enzyme at cut the DNA at a particular
site and this path is specific to each
enzyme so that is how they work thank
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