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The best procedure to improve resolution between two chromatographic peak is

  • a)
    decreasing column length, decreasing band-width

  • b)
    decreasing column length, increasing band-width

  • c)
     increasing column length, increasing band-width

  • d)
    increasing column length, decreasing band-width

Correct answer is option 'D'. Can you explain this answer?
Verified Answer
The best procedure to improve resolution between two chromatographic p...


Here are the standard ways to improve resolution:




  1. Increasing Column Length: This generally leads to better separation because it allows more time for the different components to separate.



  2. Decreasing Band-Width (Peak Width): Narrower peaks mean less overlap and thus better resolution. This can be achieved by improving the efficiency of the column through factors such as packing material, particle size, and temperature control.


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Most Upvoted Answer
The best procedure to improve resolution between two chromatographic p...
Introduction:
The resolution between two chromatographic peaks is a measure of how well-defined and separated they are. It determines the ability to distinguish between two closely eluting compounds in a chromatogram. The higher the resolution, the better the separation.

Explanation:
To improve resolution between two chromatographic peaks, it is important to consider two key factors: column length and band-width.

Column Length:
- The column length refers to the physical length of the chromatographic column, which is packed with the stationary phase.
- Increasing the column length can improve resolution because it allows for more time for the compounds to interact with the stationary phase.
- As a result, the compounds spend more time in the column, leading to better separation and resolution between the peaks.

Band-width:
- The band-width refers to the width of the peak obtained in a chromatogram.
- Decreasing the band-width improves resolution because it reduces the overlap between the two peaks.
- A narrower band-width means that the compounds spend less time eluting from the column, resulting in less broadening of the peaks.
- This leads to better separation and resolution between the peaks.

The Correct Approach:
The correct approach to improve resolution between two chromatographic peaks is to simultaneously decrease the column length and the band-width.

Decreasing Column Length:
- Decreasing the column length reduces the time it takes for the compounds to elute from the column.
- This results in shorter retention times and faster elution, which can improve the peak separation and resolution.
- However, it is important to note that decreasing the column length too much may compromise the efficiency and resolution of the separation.

Decreasing Band-width:
- Decreasing the band-width involves optimizing the chromatographic conditions to minimize peak broadening.
- This can be achieved by using smaller particle sizes in the stationary phase, which leads to narrower peaks.
- Additionally, optimizing the flow rate and injection volume can also help reduce band-width.
- By decreasing the band-width, the overlap between the peaks is minimized, leading to improved resolution.

Conclusion:
In conclusion, the best procedure to improve resolution between two chromatographic peaks is to decrease the column length and decrease the band-width. This approach allows for faster elution and reduces peak broadening, resulting in better separation and resolution between the peaks.
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The best procedure to improve resolution between two chromatographic peak isa)decreasing column length, decreasing band-widthb)decreasing column length, increasing band-widthc)increasing column length, increasing band-widthd)increasing column length, decreasing band-widthCorrect answer is option 'D'. Can you explain this answer?
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