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A.    Protein moved more slowly in an SDS- PAGE.
B.    Isoelectric focusing [IEF] showed that there was no change in the pI
C.    Mass spectrometric analysis showed that the modification was on serine
The modification that the protein undergoes is likely to be
  • a)
    phosphorylation
  • b)
    glycosylation
  • c)
    ubiquitination
  • d)
    ADP- ribosylation
Correct answer is option 'B'. Can you explain this answer?
Verified Answer
A. Protein moved more slowly in an SDS- PAGE.B. Isoelectric focusing [...
Since, there is no change in the pI after post translational modification, it means the post translational modification cannot be phosphorylation, ADP ribosylation or ubiquitination as these three modifications will change the ionization state of protein and hence change the pI so on IEF their location will be different. Therefore the given protein has glycosylation modification. Also serine cannot undergo ubiquitination; ubiquitination occurs on lysine residue.
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A. Protein moved more slowly in an SDS- PAGE.B. Isoelectric focusing [IEF] showed that there was no change in the pIC. Mass spectrometric analysis showed that the modification was on serineThe modification that the protein undergoes is likely to bea)phosphorylationb)glycosylationc)ubiquitinationd)ADP- ribosylationCorrect answer is option 'B'. Can you explain this answer?
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A. Protein moved more slowly in an SDS- PAGE.B. Isoelectric focusing [IEF] showed that there was no change in the pIC. Mass spectrometric analysis showed that the modification was on serineThe modification that the protein undergoes is likely to bea)phosphorylationb)glycosylationc)ubiquitinationd)ADP- ribosylationCorrect answer is option 'B'. Can you explain this answer? for IIT JAM 2024 is part of IIT JAM preparation. The Question and answers have been prepared according to the IIT JAM exam syllabus. Information about A. Protein moved more slowly in an SDS- PAGE.B. Isoelectric focusing [IEF] showed that there was no change in the pIC. Mass spectrometric analysis showed that the modification was on serineThe modification that the protein undergoes is likely to bea)phosphorylationb)glycosylationc)ubiquitinationd)ADP- ribosylationCorrect answer is option 'B'. Can you explain this answer? covers all topics & solutions for IIT JAM 2024 Exam. Find important definitions, questions, meanings, examples, exercises and tests below for A. Protein moved more slowly in an SDS- PAGE.B. Isoelectric focusing [IEF] showed that there was no change in the pIC. Mass spectrometric analysis showed that the modification was on serineThe modification that the protein undergoes is likely to bea)phosphorylationb)glycosylationc)ubiquitinationd)ADP- ribosylationCorrect answer is option 'B'. Can you explain this answer?.
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