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Why dideoxynucleoside triphosphates (ddNTPs) are used in DNA sequencing?
  • a)
    Because ddNTPs prevent further DNA synthesis once they are incorporated into the DNA sequence.
  • b)
    Because ddNTPs get incorporated into DNA by an enzyme DNA polymerase very efficiently.
  • c)
    Because ddNTPs are fluorescent.
  • d)
    All of the above.
Correct answer is option 'A'. Can you explain this answer?
Verified Answer
Why dideoxynucleoside triphosphates (ddNTPs) are used in DNA sequencin...
Dideoxy derivatives of nucleoside triphosphates (ddNTPs) are used in DNA sequencing, because if one of these molecules is added to a growing DNA chain, synthesis stops. This is because DNA polymerase adds a nucleotide to the 3’-OH of a growing DNA chain and ddNTPs lack the 3’-OH group. Hence, although they can be added to a chain via their 5’-phosphate, the chain is then terminated. In sequencing reaction the fraction of newly synthesized DNA chains that are terminated will depend on the relative proportions of dNTP and ddNTP.
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Why dideoxynucleoside triphosphates (ddNTPs) are used in DNA sequencing?a)Because ddNTPs prevent further DNA synthesis once they are incorporated into the DNA sequence.b)Because ddNTPs get incorporated into DNA by an enzyme DNA polymerase very efficiently.c)Because ddNTPs are fluorescent.d)All of the above.Correct answer is option 'A'. Can you explain this answer?
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